Molecular characterization of the t(8; 13)(p11;q12) translocation associated with an atypical myeloproliferative disorder: Evidence for three discrete loci involved in myeloid leukemias on 8p11

Ivan H. Still, Olga Chernova, David Hurd, Richard M. Stone, John Kenneth Cowell

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Abstract

A reciprocal chromosome translocation between 13q12 and 8p11 is the consistent cytogenetic abnormality seen in a nonspecific myeloproliferative disorder that is associated with T-cell leukemia/lymphoma and peripheral blood eosinophilia. Detailed molecular analyses of the translocation breakpoints associated with this rearrangement have not been reported to date. We have now generated somatic cell hybrids from a newly described patient with this specific structural rearrangement and analyzed the breakpoints on the derivative chromosomes. We have shown that the breakpoint on chromosome 13 lies within a 300- to 500-kb region defined by the KIAA177 gene and D13S1123 marker. In addition, we have identified a 1.2-Mb YAC, 959A4, that crosses the translocation breakpoint on the short arm of chromosome 8 in this patient. The location of this breakpoint in 8p11 is distinct from the t(8; 16) and t(8; 22) translocations associated with M4/M5 myeloid leukemias, and suggests that three distinct loci located within 8p11 are involved in the pathogenesis of myeloid neoplasias.

Original languageEnglish (US)
Pages (from-to)3136-3141
Number of pages6
JournalBlood
Volume90
Issue number8
StatePublished - Oct 15 1997
Externally publishedYes

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Myeloproliferative Disorders
Myeloid Leukemia
Chromosomes
T-Cell Leukemia
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 8
Hybrid Cells
T-Cell Lymphoma
Eosinophilia
Chromosome Aberrations
T-cells
Genes
Neoplasms
Blood
Derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Molecular characterization of the t(8; 13)(p11;q12) translocation associated with an atypical myeloproliferative disorder : Evidence for three discrete loci involved in myeloid leukemias on 8p11. / Still, Ivan H.; Chernova, Olga; Hurd, David; Stone, Richard M.; Cowell, John Kenneth.

In: Blood, Vol. 90, No. 8, 15.10.1997, p. 3136-3141.

Research output: Contribution to journalArticle

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abstract = "A reciprocal chromosome translocation between 13q12 and 8p11 is the consistent cytogenetic abnormality seen in a nonspecific myeloproliferative disorder that is associated with T-cell leukemia/lymphoma and peripheral blood eosinophilia. Detailed molecular analyses of the translocation breakpoints associated with this rearrangement have not been reported to date. We have now generated somatic cell hybrids from a newly described patient with this specific structural rearrangement and analyzed the breakpoints on the derivative chromosomes. We have shown that the breakpoint on chromosome 13 lies within a 300- to 500-kb region defined by the KIAA177 gene and D13S1123 marker. In addition, we have identified a 1.2-Mb YAC, 959A4, that crosses the translocation breakpoint on the short arm of chromosome 8 in this patient. The location of this breakpoint in 8p11 is distinct from the t(8; 16) and t(8; 22) translocations associated with M4/M5 myeloid leukemias, and suggests that three distinct loci located within 8p11 are involved in the pathogenesis of myeloid neoplasias.",
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