Molecular characterization of the t(8; 13)(p11;q12) translocation associated with an atypical myeloproliferative disorder

Evidence for three discrete loci involved in myeloid leukemias on 8p11

Ivan H. Still, Olga Chernova, David Hurd, Richard M. Stone, John Kenneth Cowell

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A reciprocal chromosome translocation between 13q12 and 8p11 is the consistent cytogenetic abnormality seen in a nonspecific myeloproliferative disorder that is associated with T-cell leukemia/lymphoma and peripheral blood eosinophilia. Detailed molecular analyses of the translocation breakpoints associated with this rearrangement have not been reported to date. We have now generated somatic cell hybrids from a newly described patient with this specific structural rearrangement and analyzed the breakpoints on the derivative chromosomes. We have shown that the breakpoint on chromosome 13 lies within a 300- to 500-kb region defined by the KIAA177 gene and D13S1123 marker. In addition, we have identified a 1.2-Mb YAC, 959A4, that crosses the translocation breakpoint on the short arm of chromosome 8 in this patient. The location of this breakpoint in 8p11 is distinct from the t(8; 16) and t(8; 22) translocations associated with M4/M5 myeloid leukemias, and suggests that three distinct loci located within 8p11 are involved in the pathogenesis of myeloid neoplasias.

Original languageEnglish (US)
Pages (from-to)3136-3141
Number of pages6
JournalBlood
Volume90
Issue number8
StatePublished - Oct 15 1997
Externally publishedYes

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Myeloproliferative Disorders
Myeloid Leukemia
Chromosomes
T-Cell Leukemia
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 8
Hybrid Cells
T-Cell Lymphoma
Eosinophilia
Chromosome Aberrations
T-cells
Genes
Neoplasms
Blood
Derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Molecular characterization of the t(8; 13)(p11;q12) translocation associated with an atypical myeloproliferative disorder : Evidence for three discrete loci involved in myeloid leukemias on 8p11. / Still, Ivan H.; Chernova, Olga; Hurd, David; Stone, Richard M.; Cowell, John Kenneth.

In: Blood, Vol. 90, No. 8, 15.10.1997, p. 3136-3141.

Research output: Contribution to journalArticle

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abstract = "A reciprocal chromosome translocation between 13q12 and 8p11 is the consistent cytogenetic abnormality seen in a nonspecific myeloproliferative disorder that is associated with T-cell leukemia/lymphoma and peripheral blood eosinophilia. Detailed molecular analyses of the translocation breakpoints associated with this rearrangement have not been reported to date. We have now generated somatic cell hybrids from a newly described patient with this specific structural rearrangement and analyzed the breakpoints on the derivative chromosomes. We have shown that the breakpoint on chromosome 13 lies within a 300- to 500-kb region defined by the KIAA177 gene and D13S1123 marker. In addition, we have identified a 1.2-Mb YAC, 959A4, that crosses the translocation breakpoint on the short arm of chromosome 8 in this patient. The location of this breakpoint in 8p11 is distinct from the t(8; 16) and t(8; 22) translocations associated with M4/M5 myeloid leukemias, and suggests that three distinct loci located within 8p11 are involved in the pathogenesis of myeloid neoplasias.",
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