Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1)

Jing Da Shi, Thomas Kukar, Cong Yi Wang, Quan Zhen Li, Pedro E. Cruz, Abdoreza Davoodi-Semiromi, Ping Yang, Yunrong Gu, Wei Lian, Donghai H. Wu, Jin-Xiong She

Research output: Contribution to journalArticle

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Abstract

Here we describe the cloning, localization, and characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse. The predicted human LALP1 gene encodes a 604-amino acid protein, whereas the mouse Lalp1 gene encodes a 606-amino acid protein. The human and mouse genes have 88% amino acid sequence identity. These genes share considerable homologies with hLALP70, a recently discovered mammalian lysosomal endo-apyrase. The human LALP1 gene resides on chromosome 10q23-q24 and contains 12 exons and 11 introns covering a genomic region of ∼46 kilobase pairs. The subcellular localization and enzymatic activity of LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, GTP, and CTP.

Original languageEnglish (US)
Pages (from-to)17474-17478
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number20
DOIs
StatePublished - May 18 2001
Externally publishedYes

Fingerprint

Apyrase
Cloning
Molecular Cloning
Genes
Amino Acids
Cytidine Triphosphate
Uridine Triphosphate
Chromosomes
Guanosine Triphosphate
Nucleosides
Introns
Organism Cloning
Amino Acid Sequence
Exons
Proteins
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Shi, J. D., Kukar, T., Wang, C. Y., Li, Q. Z., Cruz, P. E., Davoodi-Semiromi, A., ... She, J-X. (2001). Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1). Journal of Biological Chemistry, 276(20), 17474-17478. https://doi.org/10.1074/jbc.M011569200

Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1). / Shi, Jing Da; Kukar, Thomas; Wang, Cong Yi; Li, Quan Zhen; Cruz, Pedro E.; Davoodi-Semiromi, Abdoreza; Yang, Ping; Gu, Yunrong; Lian, Wei; Wu, Donghai H.; She, Jin-Xiong.

In: Journal of Biological Chemistry, Vol. 276, No. 20, 18.05.2001, p. 17474-17478.

Research output: Contribution to journalArticle

Shi, JD, Kukar, T, Wang, CY, Li, QZ, Cruz, PE, Davoodi-Semiromi, A, Yang, P, Gu, Y, Lian, W, Wu, DH & She, J-X 2001, 'Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1)', Journal of Biological Chemistry, vol. 276, no. 20, pp. 17474-17478. https://doi.org/10.1074/jbc.M011569200
Shi JD, Kukar T, Wang CY, Li QZ, Cruz PE, Davoodi-Semiromi A et al. Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1). Journal of Biological Chemistry. 2001 May 18;276(20):17474-17478. https://doi.org/10.1074/jbc.M011569200
Shi, Jing Da ; Kukar, Thomas ; Wang, Cong Yi ; Li, Quan Zhen ; Cruz, Pedro E. ; Davoodi-Semiromi, Abdoreza ; Yang, Ping ; Gu, Yunrong ; Lian, Wei ; Wu, Donghai H. ; She, Jin-Xiong. / Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1). In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 20. pp. 17474-17478.
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