Molecular cloning and characterization of alc the gene encoding allantoicase of Neurospora crassa

Hakjoo Lee, Ying Hui Fu, George A. Marzluf

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Purrtins can be utilized as a secondary nitrogen source by Neurospora crassa during conditions of nitrogen limitation. The expression of purine catabolic enzymes is governed by the nitrogen regulatory circuit and requires induction by uric acid. The major positive-acting nitrogen regulatory gene, nit-2, turns on the expression of the purine catabolic enzymes, which may also be subject to negative regulation by a second control gene, nmr. We have cloned alc, the structural gene which encodes allantoicase, an inducible enzyme of the purine degradative pathway. The identity of the alc clone was confirmed by restriction fragment length polymorphism analysis and by repeat-induced mutation. The alc gene is transcribed to give a single messenger RNA, approximately 1.2 kb in length. The negative-acting nmr gene affects the expression of alc in the expected manner. Both the nit-2 and the nmr control genes affect alc mRNA levels and allantoicase enzyme activity in both the induced and nitrogen-repressed conditions.

Original languageEnglish (US)
Pages (from-to)140-144
Number of pages5
JournalMGG Molecular & General Genetics
Volume222
Issue number1
DOIs
StatePublished - Jun 1 1990
Externally publishedYes

Keywords

  • Allantoicase
  • Neurospora crassa
  • Nitrogen regulation
  • Purine catabolic pathway

ASJC Scopus subject areas

  • Genetics

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