Molecular cloning and expression of mouse brain sialidase

Christian L. Fronda, Guichao Zeng, Luoyi Gao, Robert K Yu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Sialidase (EC 3.2.1.18) catalyzes the release of sialic acid from sialo-oligosaccharides, gangliosides, or sialo-glycoproteins. In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells. This 1699 bp cDNA codes for a 41.6 kDa protein consisting of 372 deduced amino acid residues. In COS-7 cells transiently transfected with the cDNA, a 250-fold increase was observed in specific activity toward 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid. Similarity searches of the nonredundant GenBank peptide sequence database by the PSI-BLAST program identified rat, hamster, human, and bacterial sialidases homologous to this mouse brain sialidase. Amino acid sequence identities to rat and hamster sialidases (84% and 77%, respectively) suggest that this form of sialidase is conserved in rodents. Sequence identities to human and mouse lysosomal sialidases (30% and 28%, respectively) indicate that the mouse brain sialidase is distinct from the lysosomal enzyme. Mouse brain sialidase has two amino acid sequence motifs common to bacterial sialidases: the 'F/YRIP' motif and the 'Asp-box' motif. The 'F/YRIP' motif is present near the N terminus while two 'Asp-box' motifs are present downstream.

Original languageEnglish (US)
Pages (from-to)727-731
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume258
Issue number3
DOIs
StatePublished - May 19 1999
Externally publishedYes

Fingerprint

Cloning
Neuraminidase
Molecular Cloning
Brain
Complementary DNA
COS Cells
N-Acetylneuraminic Acid
Amino Acids
Cricetinae
Rats
Amino Acid Sequence
Amino Acid Motifs
Gangliosides
Nucleic Acid Databases
Oligosaccharides
Rodentia
Glycoproteins
Databases
Peptides
Enzymes

Keywords

  • Sialidase
  • cDNA expression
  • cDNA sequence

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning and expression of mouse brain sialidase. / Fronda, Christian L.; Zeng, Guichao; Gao, Luoyi; Yu, Robert K.

In: Biochemical and Biophysical Research Communications, Vol. 258, No. 3, 19.05.1999, p. 727-731.

Research output: Contribution to journalArticle

Fronda, Christian L. ; Zeng, Guichao ; Gao, Luoyi ; Yu, Robert K. / Molecular cloning and expression of mouse brain sialidase. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 258, No. 3. pp. 727-731.
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