Molecular cloning and expression of palmitoyl-protein thioesterase

Laura A. Camp, Linda A. Verkruyse, Steven J. Afendis, Clive A. Slaughter, Sandra L. Hofmann

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

We have previously reported the purification of a palmitoyl-protein thioesterase (PPT) from bovine brain that removes palmitate from Ha-Ras (Camp, L. A., and Hofmann, S. L. (1993) J. Biol. Chem. 268, 22566-22574). In the current paper, we have isolated bovine and rat cDNA clones encoding PPT. The deduced amino acid sequence of PPT predicts a protein of 306 amino acids that contains amino acid motifs characteristic of thioesterases: 'Gly-X-Ser- X-Gly' positioned near the NH2 terminus and 'Gly-Asp-His' positioned near the COOH terminus of the protein. The identity of the PPT cDNA was further confirmed by expression in simian COS cells and insect Sf9 cells. Comparison of the DNA and protein sequence data suggests that a hydrophobic NH2- terminal sequence of 27 amino acid residues is removed from the primary translation product. Furthermore, the recombinant protein and the native protein purified from bovine brain contain complex asparagine-linked oligosaccharides and a large proportion of the expressed PPT is secreted from COS and Sf9 cells. Thus, while the palmitoyl-protein thioesterase will deacylate intracellular palmitoylated proteins such as Ha-Ras and the α subunits of heterotrimeric G proteins, the physiologic substrates are likely to be externally oriented or secreted proteins.

Original languageEnglish (US)
Pages (from-to)23212-23219
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number37
StatePublished - Sep 16 1994
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Sf9 Cells
Amino Acids
Proteins
COS Cells
Amino Acid Sequence
Brain
Complementary DNA
Heterotrimeric GTP-Binding Proteins
Amino Acid Motifs
Palmitates
Asparagine
Oligosaccharides
Recombinant Proteins
Purification
Insects
palmitoyl-protein thioesterase
Rats
Clone Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Camp, L. A., Verkruyse, L. A., Afendis, S. J., Slaughter, C. A., & Hofmann, S. L. (1994). Molecular cloning and expression of palmitoyl-protein thioesterase. Journal of Biological Chemistry, 269(37), 23212-23219.

Molecular cloning and expression of palmitoyl-protein thioesterase. / Camp, Laura A.; Verkruyse, Linda A.; Afendis, Steven J.; Slaughter, Clive A.; Hofmann, Sandra L.

In: Journal of Biological Chemistry, Vol. 269, No. 37, 16.09.1994, p. 23212-23219.

Research output: Contribution to journalArticle

Camp, LA, Verkruyse, LA, Afendis, SJ, Slaughter, CA & Hofmann, SL 1994, 'Molecular cloning and expression of palmitoyl-protein thioesterase', Journal of Biological Chemistry, vol. 269, no. 37, pp. 23212-23219.
Camp LA, Verkruyse LA, Afendis SJ, Slaughter CA, Hofmann SL. Molecular cloning and expression of palmitoyl-protein thioesterase. Journal of Biological Chemistry. 1994 Sep 16;269(37):23212-23219.
Camp, Laura A. ; Verkruyse, Linda A. ; Afendis, Steven J. ; Slaughter, Clive A. ; Hofmann, Sandra L. / Molecular cloning and expression of palmitoyl-protein thioesterase. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 37. pp. 23212-23219.
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