Molecular cloning of Aplysia neuronal cDNAs that encode carboxypeptidases related to mammalian prohormone processing enzymes

Xuemo Fan, Gregg T. Nagle

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The bag cell neurons of Aplysia synthesize an egg-laying hormone (ELH) precursor that initially is cleaved into two fragments in the Golgi apparatus, and the fragments are differentially packaged in separate granule populations and further processed. Aplysia Afurin, Afurin2, prohormone convertase 1 (PC1), and PC2 are thought to be involved in the posttranslational processing of the ELH prohormone. In the present study, we have cloned Aplysia neuronal cDNAs that encode an enzyme most closely related to mammalian carboxypeptidase E (CPE), a peptide hormone processing enzyme that removes basic residues during prohormone processing. Northern blot analysis identified a single Aplysia CPE mRNA (~5.2 kb) in central nervous system tissue. The C-terminal region of Aplysia CPE contains amphiphilic α- helices that may serve as a hydrophobic membrane anchor. A novel neuronal Aplysia enzyme was also identified by the polymerase chain reaction that was most closely related to the carboxypeptidase D (CPD)-related duck protein gp180 and the Drosophila silver gene carboxypeptidases. Aplysia CPE and the CPD-related enzyme are candidate processing enzymes that may play a role in the processing of the ELH prohormone and other Aplysia prohormones.

Original languageEnglish (US)
Pages (from-to)937-945
Number of pages9
JournalDNA and Cell Biology
Volume15
Issue number11
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Carboxypeptidases
Aplysia
Molecular Cloning
Carboxypeptidase H
Complementary DNA
Enzymes
4 alpha-glucanotransferase
Ovum
Proprotein Convertase 2
Proprotein Convertase 1
Hormones
Nerve Tissue
Ducks
Peptide Hormones
Golgi Apparatus
Northern Blotting
Central Nervous System
Neurons
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Molecular cloning of Aplysia neuronal cDNAs that encode carboxypeptidases related to mammalian prohormone processing enzymes. / Fan, Xuemo; Nagle, Gregg T.

In: DNA and Cell Biology, Vol. 15, No. 11, 01.01.1996, p. 937-945.

Research output: Contribution to journalArticle

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