Abstract
The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.
Original language | English (US) |
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Pages (from-to) | 319-323 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 226 |
Issue number | 2 |
DOIs | |
State | Published - Sep 13 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology