Molecular cloning of cDNA for rat brain GD3-synthase

Guichao Zeng, Luoyi Gao, Toshio Ariga, Robert K Yu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number2
DOIs
StatePublished - Sep 13 1996
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Rats
Brain
Complementary DNA
Antisense Oligonucleotides
Membrane Potentials
Neurons
Amplification
Amino Acid Sequence
Nucleotides
Amino Acids
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning of cDNA for rat brain GD3-synthase. / Zeng, Guichao; Gao, Luoyi; Ariga, Toshio; Yu, Robert K.

In: Biochemical and Biophysical Research Communications, Vol. 226, No. 2, 13.09.1996, p. 319-323.

Research output: Contribution to journalArticle

Zeng, Guichao ; Gao, Luoyi ; Ariga, Toshio ; Yu, Robert K. / Molecular cloning of cDNA for rat brain GD3-synthase. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 226, No. 2. pp. 319-323.
@article{dad27924559e4dcea9165ca0300e411e,
title = "Molecular cloning of cDNA for rat brain GD3-synthase",
abstract = "The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.",
author = "Guichao Zeng and Luoyi Gao and Toshio Ariga and Yu, {Robert K}",
year = "1996",
month = "9",
day = "13",
doi = "10.1006/bbrc.1996.1354",
language = "English (US)",
volume = "226",
pages = "319--323",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular cloning of cDNA for rat brain GD3-synthase

AU - Zeng, Guichao

AU - Gao, Luoyi

AU - Ariga, Toshio

AU - Yu, Robert K

PY - 1996/9/13

Y1 - 1996/9/13

N2 - The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.

AB - The complete nucleotide sequence of the cDNA coding for rat GD3-synthase was determined for the first time using PCR and the rapid amplification of cDNA ends (RACE) procedures. The deduced amino acid sequence consists of 359 residues. The protein contains a potential transmembrane domain with a short N-terminus and such highly conserved regions as the sialylmotifs. An antisense oligonucleotide derived from the rat GD3-synthase cDNA was applied to the cultures of rat cerebellar neurons and results showed a decrease in the synthesis of ganglioside GD3, indicating that the expression of GD3-synthase was modulated by the antisense sequence.

UR - http://www.scopus.com/inward/record.url?scp=0030582383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030582383&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.1354

DO - 10.1006/bbrc.1996.1354

M3 - Article

VL - 226

SP - 319

EP - 323

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -