Molecular cloning of the human placental folate transporter

Puttur D Prasad, Sammanda Ramamoorthy, Frederick H. Lei Bach, Vadivel Ganapathy

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

We have isolated a cDNA clone from a human placental library which codes for a functional folate transporter (hFOLT). An RT-PCR product obtained from mouse intestinal mRNA using primers specific for the mouse folate transporter cDNA was used to screen the cDNA library. The hFOLT cDNA predicts a protein of 590 amino acids with twelve potential transmembrane domains. The amino acid sequence has 65% identity to the mouse and hamster folate transporters. When transfected into COS-1 and HeLa cells, the hFOLT cDNA causes a significant increase in the uptake of 5-methyltetrahydrofolate. The mRNA transcripts hybridizing to the hFOLT cDNA are detectable in the placenta and liver and also in several cell lines of human origin. The size of the principal transcript is 2.7 kb. This constitutes the first report on the cloning of a vitamin transporter from a human tissue.

Original languageEnglish (US)
Pages (from-to)681-687
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume206
Issue number2
DOIs
StatePublished - Jan 17 1995

Fingerprint

Folic Acid Transporters
Cloning
Molecular Cloning
Complementary DNA
Amino Acids
Messenger RNA
COS Cells
Gene Library
HeLa Cells
Vitamins
Cricetinae
Liver
Membrane Potentials
Placenta
Libraries
Organism Cloning
Amino Acid Sequence
Clone Cells
Cells
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning of the human placental folate transporter. / Prasad, Puttur D; Ramamoorthy, Sammanda; Lei Bach, Frederick H.; Ganapathy, Vadivel.

In: Biochemical and Biophysical Research Communications, Vol. 206, No. 2, 17.01.1995, p. 681-687.

Research output: Contribution to journalArticle

Prasad, Puttur D ; Ramamoorthy, Sammanda ; Lei Bach, Frederick H. ; Ganapathy, Vadivel. / Molecular cloning of the human placental folate transporter. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 206, No. 2. pp. 681-687.
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