Molecular cloning of the human placental folate transporter

Puttur D. Prasad, Sammanda Ramamoorthy, Frederick H. Lei Bach, Vadivel Ganapathy

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

We have isolated a cDNA clone from a human placental library which codes for a functional folate transporter (hFOLT). An RT-PCR product obtained from mouse intestinal mRNA using primers specific for the mouse folate transporter cDNA was used to screen the cDNA library. The hFOLT cDNA predicts a protein of 590 amino acids with twelve potential transmembrane domains. The amino acid sequence has 65% identity to the mouse and hamster folate transporters. When transfected into COS-1 and HeLa cells, the hFOLT cDNA causes a significant increase in the uptake of 5-methyltetrahydrofolate. The mRNA transcripts hybridizing to the hFOLT cDNA are detectable in the placenta and liver and also in several cell lines of human origin. The size of the principal transcript is 2.7 kb. This constitutes the first report on the cloning of a vitamin transporter from a human tissue.

Original languageEnglish (US)
Pages (from-to)681-687
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume206
Issue number2
DOIs
StatePublished - Jan 17 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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