TY - JOUR
T1 - Molecular detection of B-cell neoplasms by specific DNA methylation biomarkers
AU - Wang, Michael X.
AU - Wang, Huan You
AU - Zhao, Xiaohui
AU - Srilatha, Nalluri
AU - Zheng, Dali
AU - Shi, Huidong
AU - Ning, Jie
AU - Duff, Deiter J.
AU - Taylor, Kristen H.
AU - Gruner, Barbara A.
AU - Caldwell, Charles W.
PY - 2010
Y1 - 2010
N2 - A novel, easy to perform PCR-based method employing specific DNA methylation biomarkers to detect Bcell neoplasms in a variety of B-cell lines and B lymphoblastic leukemia (B-ALL) patient specimens has been developed. This method detects as few as 5 B-ALL cells, or 1 B-ALL cell in 1,000,000 normal background blood cells using a single marker, DLC-1 gene CpG island (CGI) methylation. By adding two additional markers PCDHGA12 and RPIB9, over 80% of B-ALL cases were detected in patients' bone marrow and/or peripheral blood specimens. We have traced clinical B-ALL cases up to 10 years retrospectively and the DLC-1 methylation is correlated with patient clinical status. Thus, this epigenetic-based molecular method demonstrates its potential use in the diagnosis of B-cell neoplasia, in addition to traditional approach such as clinical features, morphology, immunophenotype, and genetic analysis.
AB - A novel, easy to perform PCR-based method employing specific DNA methylation biomarkers to detect Bcell neoplasms in a variety of B-cell lines and B lymphoblastic leukemia (B-ALL) patient specimens has been developed. This method detects as few as 5 B-ALL cells, or 1 B-ALL cell in 1,000,000 normal background blood cells using a single marker, DLC-1 gene CpG island (CGI) methylation. By adding two additional markers PCDHGA12 and RPIB9, over 80% of B-ALL cases were detected in patients' bone marrow and/or peripheral blood specimens. We have traced clinical B-ALL cases up to 10 years retrospectively and the DLC-1 methylation is correlated with patient clinical status. Thus, this epigenetic-based molecular method demonstrates its potential use in the diagnosis of B-cell neoplasia, in addition to traditional approach such as clinical features, morphology, immunophenotype, and genetic analysis.
KW - B lymphoblastic leukemia
KW - DNA methylation biomarker
KW - Mature B-cell neoplasms
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M3 - Article
C2 - 20224725
AN - SCOPUS:77953470572
SN - 1936-2625
VL - 3
SP - 265
EP - 279
JO - International Journal of Clinical and Experimental Pathology
JF - International Journal of Clinical and Experimental Pathology
IS - 3
ER -