Molecular genetic analysis of chromosome 11p in familial Wilms tumour

P. N. Baird, J. Pritchard, J. K. Cowell

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the family reported here, a mother and both of her children developed a Wilms tumour, and all three tumours were of the relatively rare monomorphous epithelial histopathological subtype. Using restriction fragment length polymorphism analysis, both sibs were shown to inherit the same maternal allele from the 11p13 region but different maternal alleles from the 11p15 region. Using a combination of single-strand conformation polymorphism (SSCP) and polymerase chain reaction (PCR) sequencing techniques, no mutations were identified in the WT1 tumour-suppressor gene from the 11p13 region, but a novel polymorphism was identified in exon 1. mRNA expression studies using the insulin-like growth factor II (IGF-II) gene, located in 11p15, showed that there was no relaxation of imprinting at this locus. There was also no evidence of loss of heterozygosity on the long arm of chromosome 16. These findings indicate that the WT1 and IGF-II genes, together with the long arm of chromosome 16, are not directly implicated in tumorigenesis in this Wilms family, but that a recombination event has occurred on the short arm of chromosome 11.

Original languageEnglish (US)
Pages (from-to)1072-1077
Number of pages6
JournalBritish Journal of Cancer
Volume69
Issue number6
DOIs
StatePublished - Jun 1994

Fingerprint

Wilms Tumor
Chromosomes, Human, Pair 16
Molecular Biology
Insulin-Like Growth Factor II
Chromosomes
Mothers
Alleles
Chromosomes, Human, Pair 11
Loss of Heterozygosity
Tumor Suppressor Genes
Restriction Fragment Length Polymorphisms
Genetic Recombination
Genes
Exons
Carcinogenesis
Polymerase Chain Reaction
Messenger RNA
Mutation
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Molecular genetic analysis of chromosome 11p in familial Wilms tumour. / Baird, P. N.; Pritchard, J.; Cowell, J. K.

In: British Journal of Cancer, Vol. 69, No. 6, 06.1994, p. 1072-1077.

Research output: Contribution to journalArticle

Baird, P. N. ; Pritchard, J. ; Cowell, J. K. / Molecular genetic analysis of chromosome 11p in familial Wilms tumour. In: British Journal of Cancer. 1994 ; Vol. 69, No. 6. pp. 1072-1077.
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