Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia

S. F. Cummins, L. Leblanc, B. M. Degnan, Gregg Thomas Nagle

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An ability to sense and respond to environmental cues is essential to the survival of most marine animals. How water-borne chemical cues are detected at the molecular level and processed by molluscs is currently unknown. In this study, we cloned two genes from the marine mollusk Aplysia dactylomela which encode multi-transmembrane proteins. We have performed in situ hybridization that reveals expression and spatial distribution within the long-distance chemosensory organs, the rhinophores. This finding suggests that they could be receptors involved in binding water-borne chemicals and coupling to an intracellular signal pathway. In support of this, we found expression of a phospholipase C and an inositol trisphosphate receptor in the rhinophore sensory epithelia and possibly distributed within outer dendrites of olfactory sensory neurons. In Aplysia, mate attraction and subsequent reproduction is initiated by responding to a cocktail of water-borne protein pheromones released by animal conspecifics. We show that the rhinophore contraction in response to pheromone stimulants is significantly altered following phospholipase C inhibition. Overall, these data provide insight into the molecular components of chemosensory detection in a mollusk. An important next step will be the elucidation of how these coordinate the detection of chemical cues present in the marine environment and activation of sensory neurons.

Original languageEnglish (US)
Pages (from-to)2037-2044
Number of pages8
JournalJournal of Experimental Biology
Volume212
Issue number13
DOIs
StatePublished - Jul 1 2009

Fingerprint

Aplysia
chemoreceptors
Mollusca
Genetic Association Studies
molluscs
Cues
signal transduction
Signal Transduction
epithelium
Epithelium
phospholipase C
chemical cue
sensory neurons
Pheromones
Type C Phospholipases
pheromone
pheromones
Water
gene
mate attraction

Keywords

  • Aplysia
  • Inositol trisphosphate receptor
  • Pheromones
  • Phospholipase C
  • Receptor

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Molecular identification of candidate chemoreceptor genes and signal transduction components in the sensory epithelium of Aplysia. / Cummins, S. F.; Leblanc, L.; Degnan, B. M.; Nagle, Gregg Thomas.

In: Journal of Experimental Biology, Vol. 212, No. 13, 01.07.2009, p. 2037-2044.

Research output: Contribution to journalArticle

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