Molecular mimicry: Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside

Seigo Usuki, Stuart A. Thompson, Michael H. Rivner, Kyoji Taguchi, Keiko Shibata, Toshio Ariga, Robert K. Yu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Recently we have reported cases of demyelinating inflammatory neuropathy showing elevated titers of anti-GD3 antibodies, which occurs rarely in Guillain-Barré syndrome. To examine the correlation between the anti-GD3 antibody titer and Campylobacter jejuni infection, we sensitized female Lewis rats with lipopolysaccharides (LPSs) from serotype HS19 of C. jejuni and examined changes in nerve conduction velocity and nerve conduction block (P/D ratio). After 16 weeks of sensitization, animals revealed decreases of nerve conduction velocity and conduction block (P/D ratio) and high titer of anti-GD3 antibodies. These anti-GD3 antibodies also blocked transmission in neuromuscular junctions of spinal cord-muscle cells cocultures. The GD3 epitope was verified to be located on the Schwann cell surface and nodes of Ranvier in rat sciatic nerve. To determine the target epitope for GD3 antibodies in causing nerve dysfunction, the LPS fraction containing the GD3 epitope was purified from the total LPS by using an anti-GD3 monoclonal antibody-immobilized affinity column. Subsequently, chemical analysis of the oligosaccharide portion was performed and confirmed the presence of a GD3-like epitope as having the following tetrasaccharide structure: NeuAcα2-8NeuAc2-3Galβ1-4Hep. Our data thus support the possibility of a contribution of GD3 mimicry as a potential pathogenic mechanism of peripheral nerve dysfunction.

Original languageEnglish (US)
Pages (from-to)274-284
Number of pages11
JournalJournal of Neuroscience Research
Volume83
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Molecular Mimicry
Campylobacter jejuni
Antibody Formation
Lipopolysaccharides
Epitopes
Anti-Idiotypic Antibodies
Neural Conduction
Ranvier's Nodes
Campylobacter Infections
Antibody Affinity
Nerve Block
Neuromuscular Junction
Schwann Cells
Sciatic Nerve
Coculture Techniques
Oligosaccharides
Peripheral Nerves
Muscle Cells
Spinal Cord
Monoclonal Antibodies

Keywords

  • Antiganglioside antibody
  • Campylobacter jejuni
  • GD3
  • Ganglioside
  • Guillain-Barré
  • LPS
  • Syndrome

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Molecular mimicry : Sensitization of Lewis rats with Campylobacter jejuni lipopolysaccharides induces formation of antibody toward GD3 ganglioside. / Usuki, Seigo; Thompson, Stuart A.; Rivner, Michael H.; Taguchi, Kyoji; Shibata, Keiko; Ariga, Toshio; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 83, No. 2, 01.02.2006, p. 274-284.

Research output: Contribution to journalArticle

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