Molecular phylogenies in the genus Mus: Comparative analysis of electrophoretic, scnDNA hybridization, and mtDNA RFLP data

Jin-Xiong She, F. BONHOMME, P. BOURSOT, L. THALER, F. CATZEFLIS

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

In this paper, we present data on murid rodents obtained by three major techniques used in biochemical systematics today: protein electrophoresis, scnDNA hybridization and mtDNA RFLP. The data have been analysed by both phenetic and cladistic methods and results of the three techniques were compared with one another. Four clear major levels of divergence (or nodes) can be recognized by all methods. Node 1 groups four subspecies of M. musculus (domesticus, bactrianus, castaneus and musculus). Node 2 groups four species: M. musculus, M. spretus, M. spicilegus and M. spretoides. Node 3 groups the stem of node 2 and three strictly Oriental species (M. caroli, M. cervicolor and M. cookii) Node 4 groups the previous lineages with Oriental Pyromys and Coelomys and the Ethiopian Nannomys. However, the relationships within each level cannot be resolved without ambiguity. We argue that this is not due to the resolutive power of our methods, but to a biological reality, that is successive adaptive radiations marked by quasi‐simultaneous speciation events linked with geographical colonization. Our estimation of divergence time between different taxa showed that the genus Mus is very young. The youngest ‘bona fide’ species are 1.1 Myr old, or even less than 0.3 Myr if one takes into consideration the two sibling species M. spicilegus and M. spretoides. It appears that mtDNA evolves three to six times faster than scnDNA. The zoogeographical history of the genus can be reconstructed as a séries of adaptative radiations leading to the present day distribution of the Palaearctic, Oriental, and Ethiopian groups of taxa.

Original languageEnglish (US)
Pages (from-to)83-103
Number of pages21
JournalBiological Journal of the Linnean Society
Volume41
Issue number1-3
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Mus
restriction fragment length polymorphism
phylogeny
mitochondrial DNA
hybridization
divergence
adaptive radiation
stem nodes
cladistics
methodology
chemotaxonomy
rodent
subspecies
sibling species
electrokinesis
colonization
stem
electrophoresis
rodents
protein

Keywords

  • Molecular phylogeny
  • Mus‐
  • lectrophoresis
  • mtDNA RFLP
  • rate of DNA evolution
  • scnDNA hybridization

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Molecular phylogenies in the genus Mus : Comparative analysis of electrophoretic, scnDNA hybridization, and mtDNA RFLP data. / She, Jin-Xiong; BONHOMME, F.; BOURSOT, P.; THALER, L.; CATZEFLIS, F.

In: Biological Journal of the Linnean Society, Vol. 41, No. 1-3, 01.01.1990, p. 83-103.

Research output: Contribution to journalArticle

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abstract = "In this paper, we present data on murid rodents obtained by three major techniques used in biochemical systematics today: protein electrophoresis, scnDNA hybridization and mtDNA RFLP. The data have been analysed by both phenetic and cladistic methods and results of the three techniques were compared with one another. Four clear major levels of divergence (or nodes) can be recognized by all methods. Node 1 groups four subspecies of M. musculus (domesticus, bactrianus, castaneus and musculus). Node 2 groups four species: M. musculus, M. spretus, M. spicilegus and M. spretoides. Node 3 groups the stem of node 2 and three strictly Oriental species (M. caroli, M. cervicolor and M. cookii) Node 4 groups the previous lineages with Oriental Pyromys and Coelomys and the Ethiopian Nannomys. However, the relationships within each level cannot be resolved without ambiguity. We argue that this is not due to the resolutive power of our methods, but to a biological reality, that is successive adaptive radiations marked by quasi‐simultaneous speciation events linked with geographical colonization. Our estimation of divergence time between different taxa showed that the genus Mus is very young. The youngest ‘bona fide’ species are 1.1 Myr old, or even less than 0.3 Myr if one takes into consideration the two sibling species M. spicilegus and M. spretoides. It appears that mtDNA evolves three to six times faster than scnDNA. The zoogeographical history of the genus can be reconstructed as a s{\'e}ries of adaptative radiations leading to the present day distribution of the Palaearctic, Oriental, and Ethiopian groups of taxa.",
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