Molecular scanning of Yq 11 (interval 6) in men with Sertoli-cell-only syndrome

Mark D. Johnson, Sandra P.T. Tho, Ali Behzadian, Paul G McDonough

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Data suggesting that probes pDP105/B and 50f2/C,E may identify sequences on distal Yq11 (interval 6) that are critical, for spermatogenesis stimulated a study of this region by means of these two probes in azoospermic 46,XY men with biopsy-proved Sertoli-cell-only syndrome. Deoxyribonucleic acid samples from controls and study subjects were digested with the restriction enzymes Taql, EcoRl, and BamHl. These samples were blotted and hybridized with pDP105/B, 50f2/C,E, and two more proximal Yq11 probes 4B-2 and pAS1. The sequence hydridizing to 50f2/C was absent in one study subject. No deletions were detected with pDP105/B and the two more proximal probes.

Original languageEnglish (US)
Pages (from-to)1732-1737
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume161
Issue number6 PART 1
DOIs
StatePublished - Jan 1 1989

Keywords

  • DNA
  • Sertoli-cell-only syndrome
  • Spermatogenesis
  • Y chromosome

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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