Monocyte- and cytokine-induced downregulation of angiotensin-converting enzyme in cultured human and porcine endothelial cells

Andreas Papapetropoulos, Alexander Antonov, Renu Virmani, Frank D. Kolodgie, David H Munn, Nandor Marczin, James W. Ryan, Ross G. Gerrity, John D. Catravas

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19 Scopus citations

Abstract

We investigated the effects of monocytes on endothelial cell (EC) ectoenzyme activity. Coculture of human aortic ECs with human monocytes (2 x 105 monocytes per 2-cm2 well) led to a decrease in EC angiotensin- converting enzyme (ACE) activity (64.5±3.5% of control) but not aminopeptidase N, aminopeptidase P, and 5'-nucleotidase activities. Similar results were obtained using human umbilical vein EC-human monocyte and porcine aortic EC-porcine monocyte cocultures. The decrease in ACE activity was monocyte concentration and coculture time dependent, reaching a maximum of 65% decrease in activity at 120 hours. Monocyte-mediated reduction in ACE activity did not require cell to cell contact, since exposure of ECs to conditioned medium from cocultures (CCCM) or from monocyte cultures (MCM) produced a decrease in ACE activity similar to that observed in EC-monocyte cocultures. Exogenously added tumor necrosis factor (TNF)-α and interleukin (IL)-1α, two known secretory products of monocytes, simulated the effects of monocytes on ACE activity. Western blot analysis revealed a decrease in the amount of ACE protein in TNF-α-treated and CCCM-treated ECs compared with control ECs. Both TNF-α and IL-1α were present in CCCM and MCM but not EC- conditioned medium. Incubation of the cocultures with a mixture of neutralizing antibodies against TNF-α and IL-1 totally abolished the monocyte-induced decrease in ACE activity. In conclusion, monocytes decrease ACE activity in cultured ECs through the release of cytokines such as TNF-α and IL-1.

Original languageEnglish (US)
Pages (from-to)512-523
Number of pages12
JournalCirculation Research
Volume79
Issue number3
DOIs
StatePublished - Jan 1 1996

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Keywords

  • 5'-nucleotidase
  • angiotensin-converting enzyme
  • interleukin-1
  • surface peptidase
  • tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Papapetropoulos, A., Antonov, A., Virmani, R., Kolodgie, F. D., Munn, D. H., Marczin, N., Ryan, J. W., Gerrity, R. G., & Catravas, J. D. (1996). Monocyte- and cytokine-induced downregulation of angiotensin-converting enzyme in cultured human and porcine endothelial cells. Circulation Research, 79(3), 512-523. https://doi.org/10.1161/01.RES.79.3.512