Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms

Haitao Wu, Lin Mei

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Immunofluorescence or IF is a technique allowing the visualization of a specific protein or antigen in cells or tissues by binding a specific antibody chemically conjugated with a fluorescence dye. Immunofluorescent staining is widely used in life science research, particularly for neuroscience. Here, we describe the immunofluorescent staining of whole-mount neonatal mouse diaphragms to study the morphological patterns of the neuromuscular junction (NMJ) by using of presynaptic neuronal marker-neurofilament (NF) and synaptophysin antibodies; postsynaptic acetylcholine receptors (AChRs) were labeled with Alexa Fluor 594-conjugated α-bungarotoxin (α-BTX). Immunofluorescence- stained diaphragms were examined under a confocal microscope.

Original languageEnglish (US)
Title of host publicationNeural Development
Subtitle of host publicationMethods and Protocols
Pages277-285
Number of pages9
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1018
ISSN (Print)1064-3745

Fingerprint

Neuromuscular Junction
Diaphragm
Fluorescent Antibody Technique
Staining and Labeling
Bungarotoxins
Synaptophysin
Intermediate Filaments
Biological Science Disciplines
Antibodies
Cholinergic Receptors
Neurosciences
Coloring Agents
Fluorescence
Antigens
Research
Proteins
Alexa594

Keywords

  • α-BTX
  • AChRs
  • Immunofluorescence
  • Light microscopy
  • Mouse diaphragms
  • Neuromuscular junction
  • Whole mount

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wu, H., & Mei, L. (2013). Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. In Neural Development: Methods and Protocols (pp. 277-285). (Methods in Molecular Biology; Vol. 1018). https://doi.org/10.1007/978-1-62703-444-9-26

Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. / Wu, Haitao; Mei, Lin.

Neural Development: Methods and Protocols. 2013. p. 277-285 (Methods in Molecular Biology; Vol. 1018).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wu, H & Mei, L 2013, Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. in Neural Development: Methods and Protocols. Methods in Molecular Biology, vol. 1018, pp. 277-285. https://doi.org/10.1007/978-1-62703-444-9-26
Wu H, Mei L. Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. In Neural Development: Methods and Protocols. 2013. p. 277-285. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-444-9-26
Wu, Haitao ; Mei, Lin. / Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. Neural Development: Methods and Protocols. 2013. pp. 277-285 (Methods in Molecular Biology).
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