@inbook{ba43adf7a7934a73a6c2fde167daefe5,
title = "Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms",
abstract = "Immunofluorescence or IF is a technique allowing the visualization of a specific protein or antigen in cells or tissues by binding a specific antibody chemically conjugated with a fluorescence dye. Immunofluorescent staining is widely used in life science research, particularly for neuroscience. Here, we describe the immunofluorescent staining of whole-mount neonatal mouse diaphragms to study the morphological patterns of the neuromuscular junction (NMJ) by using of presynaptic neuronal marker-neurofilament (NF) and synaptophysin antibodies; postsynaptic acetylcholine receptors (AChRs) were labeled with Alexa Fluor 594-conjugated α-bungarotoxin (α-BTX). Immunofluorescence- stained diaphragms were examined under a confocal microscope.",
keywords = "AChRs, Immunofluorescence, Light microscopy, Mouse diaphragms, Neuromuscular junction, Whole mount, α-BTX",
author = "Haitao Wu and Lin Mei",
year = "2013",
doi = "10.1007/978-1-62703-444-9_26",
language = "English (US)",
isbn = "9781627034432",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "277--285",
booktitle = "Neural Development",
}