Morphological study of lectin binding in the rabbit temporomandibular joint disc

Mohamed M.H. Sharawy, A. J. Linatoc, N. L. O'dell, C. B. Pennington, V. B. Larke, Adarsh K Gulati

Research output: Contribution to journalArticle

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Abstract

Glycoconjugates of the extracellular matrix are important for the normal mechanical functions of connective tissue structures such as the temporomandibular joint disc. Since lectins are known to bind to sugar residues with high affinity, a variety of lectins were used to study the presence and distribution of glycoconjugates in the temporomandibular joint disc. Discs were removed from 6 to 8-month-old rabbits and either sectioned in a cryostat and processed for light microscopy or fixed in 2% glutaraldehyde and processed for electron microscopy. The frozen sections were incubated with fluorescein- or peroxidaseconjugated lectin solutions. Ultrathin sections mounted on grids were incubated with lectins combined with a colloidal gold marker system for electron microscopical study. Our results indicate that Canavalia ensiformis agglutinin (ConA) showed little or no binding to the discal tissue. Triticum vulgaris agglutinin (WGA) and Macluras pomifera (MPA) were bound strongly to both the synovium and the extracellular matrix and WGA also bound to the territorial matrix of chondrocyte-like cells. Glycine max and Arachis hypogoea agglutinins (SBA and PNA), were localized in the synovium and extracellular matrix but to a lesser degree than WGA and MPA. WGA, MPA, Griffonia simplicifolia II and Ulex europaeus were bound by discal fibroblasts. WGA was also localized in lysosomes of synovial A-cells (macrophages). The electron microscopical studies with lectins and colloidal gold marker systems indicated that some areas of the disc may be fibrocartilagenous as had been suggested by earlier immunohistochemical studies using monoclonal antibodies to characteristic glycosaminoglycans (GAGs) in cartilage.

Original languageEnglish (US)
Pages (from-to)132-142
Number of pages11
JournalThe Histochemical Journal
Volume23
Issue number3
DOIs
StatePublished - Mar 1 1991

Fingerprint

Temporomandibular Joint Disc
Lectins
Agglutinins
Rabbits
Extracellular Matrix
Gold Colloid
Glycoconjugates
Synovial Membrane
Canavalia
Griffonia
Electrons
Ulex
Frozen Sections
Glutaral
Chondrocytes
Lysosomes
Glycosaminoglycans
Fluorescein
Soybeans
Connective Tissue

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Morphological study of lectin binding in the rabbit temporomandibular joint disc. / Sharawy, Mohamed M.H.; Linatoc, A. J.; O'dell, N. L.; Pennington, C. B.; Larke, V. B.; Gulati, Adarsh K.

In: The Histochemical Journal, Vol. 23, No. 3, 01.03.1991, p. 132-142.

Research output: Contribution to journalArticle

Sharawy, Mohamed M.H. ; Linatoc, A. J. ; O'dell, N. L. ; Pennington, C. B. ; Larke, V. B. ; Gulati, Adarsh K. / Morphological study of lectin binding in the rabbit temporomandibular joint disc. In: The Histochemical Journal. 1991 ; Vol. 23, No. 3. pp. 132-142.
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