Mouse models for assessing the role of selenoproteins in health and development

Bradley A. Carlson, Xue Ming Xu, Rajeev Shrimali, Aniruddha Sengupta, Min Hyuk Yoo, Nianxin Zhong, Dolph L. Hatfield, Robert Irons, Cindy D. Davis, Byeong Jae Lee, Sergey V. Novoselov, Vadim N. Gladyshev

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Mouse models have been generated to assess the roles of selenoproteins involved with housekeeping tasks and/or stress-related pheno-mena in development and health. Each mouse model has taken advantage of the fact that the synthesis of all selenoproteins is dependent on the expression of two selenoeysteine (See) tRNA[Ser]Sec isoforms that differ from each other by a single methyl group on the ribosyl moiety at position 34. The endogenous Sec tRNA[SerlSsc population was selectively altered by generating mouse models involving 1) transgenic animals carrying mutant or wild type Sec tRNA[Ser]Sec transgenes, 2) conditional knockout animals carrying a floxed Sec tRNA[Ser]Sec gene that was targeted for removal in specific tissues and organs using loxP-Cre technology and 3) transgenic/standard knockout animals carrying mutant or wild type transgenes and a knockout of the Sec tRNA[Ser]Sec gene wherein the animal's survival is dependent on the transgene. These mouse models perturbed selenoprotein expression, often in a protein- and tissue-specific manner, permitting us to better assess their function in health and development.

Original languageEnglish (US)
Title of host publicationSelenium
Subtitle of host publicationIts Molecular Biology and Role in Human Health, Second Edition
PublisherSpringer US
Pages333-341
Number of pages9
ISBN (Electronic)9780387338279
ISBN (Print)0387338268, 9780387338262
DOIs
StatePublished - Jan 1 2006

ASJC Scopus subject areas

  • Medicine(all)

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    Carlson, B. A., Xu, X. M., Shrimali, R., Sengupta, A., Yoo, M. H., Zhong, N., Hatfield, D. L., Irons, R., Davis, C. D., Lee, B. J., Novoselov, S. V., & Gladyshev, V. N. (2006). Mouse models for assessing the role of selenoproteins in health and development. In Selenium: Its Molecular Biology and Role in Human Health, Second Edition (pp. 333-341). Springer US. https://doi.org/10.1007/0-387-33827-6_29