Mouse models of acute and chronic colitis

Santhakumar Manicassamy, Indumathi Manoharan

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Crohn's disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Our understanding of IBD depends largely on rodent models. DSS-induced intestinal inflammation in mice and T cell transfer colitis in SCID mice are most widely used and accepted models that can recapitulate the human diseases. Here, we provide detailed protocols of these two mouse models of experimentally induced intestinal inflammation. We also discuss the protocols for the isolation and analysis of inflammatory T cell from the colon.

Original languageEnglish (US)
Title of host publicationMouse Genetics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages437-448
Number of pages12
ISBN (Print)9781493912148
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1194
ISSN (Print)1064-3745

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Keywords

  • Acute and chronic colitis
  • DSS
  • Flow cytometry
  • IBD
  • Immune cells
  • T cell transfer colitis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Manicassamy, S., & Manoharan, I. (2014). Mouse models of acute and chronic colitis. In Mouse Genetics: Methods and Protocols (pp. 437-448). (Methods in Molecular Biology; Vol. 1194). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-1215-5_25