Mouse Models of Colitis-Associated Colon Cancer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Crohn’s disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Chronic intestinal inflammation is inextricably linked to colitis-associated colon carcinogenesis (CAC). Patients with ulcerative colitis (UC) and Crohn’s disease (CD) have an increased risk of colon cancer. Our understanding of IBD and IBD-associated colon carcinogenesis depends largely on rodent models. AOM-DSS-induced colitis-associated colon cancer in mice is the most widely used and accepted model that can recapitulate the human IBD-associated colon cancer. Here, we have provided detailed protocols of this mouse model of experimentally induced chronic intestinal inflammation-associated colon cancer. We will also discuss the protocols for the isolation and analysis of inflammatory immune cells from the colon.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages133-146
Number of pages14
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2224
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • AOM
  • CAC
  • Chronic colitis
  • DSS
  • Flow cytometry and intestinal immune cells
  • IBD-associated colon cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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