@inbook{58b80b1a6d12423e87f1f6f5efdecea1,
title = "Mouse Models of Colitis-Associated Colon Cancer",
abstract = "Crohn{\textquoteright}s disease (CD) and ulcerative colitis are two main clinically defined forms of chronic inflammatory bowel disease (IBD). Chronic intestinal inflammation is inextricably linked to colitis-associated colon carcinogenesis (CAC). Patients with ulcerative colitis (UC) and Crohn{\textquoteright}s disease (CD) have an increased risk of colon cancer. Our understanding of IBD and IBD-associated colon carcinogenesis depends largely on rodent models. AOM-DSS-induced colitis-associated colon cancer in mice is the most widely used and accepted model that can recapitulate the human IBD-associated colon cancer. Here, we have provided detailed protocols of this mouse model of experimentally induced chronic intestinal inflammation-associated colon cancer. We will also discuss the protocols for the isolation and analysis of inflammatory immune cells from the colon.",
keywords = "AOM, CAC, Chronic colitis, DSS, Flow cytometry and intestinal immune cells, IBD-associated colon cancer",
author = "Santhakumar Manicassamy and Prasad, {Puttur D.} and Daniel Swafford",
note = "Funding Information: provided by NIH (R01DK097271, R01DK123360) and Augusta University institutional development funds. Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1008-4_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "133--146",
booktitle = "Methods in Molecular Biology",
}
