Abstract
PGE2 has an established role in renal water handling. The present study was undertaken to examine the role of microsomal prostaglandin E synthase-1 (mPGES-1) in the diuretic response to acute and chronic water loading. Compared with wild-type (+/+) controls, mPGES-1 -/- mice exhibited impaired ability to excrete an acute, but not chronic water load. In response to acute water loading, urinary PGE2 excretion in the +/+ mice increased at 2 h, in parallel with increased urine flow. In contrast, the -/- mice exhibited a delayed increase in urinary PGE2 excretion, coinciding with the stimulation of renal medullary mRNA expression of cytosolic prostaglandin E synthase but not mPGES-2. At baseline, renal aquaporin-2 (AQP2) expression in mPGES-1 -/- mice was enhanced compared with the +/+ control. In response to acute water loading, renal AQP2 expression in the +/+ mice was significantly reduced, and this reduction was blunted in the -/- mice. Despite striking changes in AQP2 protein expression, renal AQP2 mRNA in both genotypes largely remained unchanged. Overall, these data support an important role of mPGES-1 in provoking the diuretic response to acute water loading.
| Original language | English (US) |
|---|---|
| Pages (from-to) | F1129-F1135 |
| Journal | American Journal of Physiology - Renal Physiology |
| Volume | 296 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1 2009 |
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Keywords
- Aquaporin-2
- Collecting duct
- Diuresis
- Water loading
ASJC Scopus subject areas
- Physiology
- Urology
Cite this
mPGES-1 deletion impairs diuretic response to acute water loading. / Soodvilai, Sunhapas; Jia, Zhanjun; Wang, Mong Heng; Dong, Zheng; Yang, Tianxin.
In: American Journal of Physiology - Renal Physiology, Vol. 296, No. 5, 01.05.2009, p. F1129-F1135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - mPGES-1 deletion impairs diuretic response to acute water loading
AU - Soodvilai, Sunhapas
AU - Jia, Zhanjun
AU - Wang, Mong Heng
AU - Dong, Zheng
AU - Yang, Tianxin
PY - 2009/5/1
Y1 - 2009/5/1
N2 - PGE2 has an established role in renal water handling. The present study was undertaken to examine the role of microsomal prostaglandin E synthase-1 (mPGES-1) in the diuretic response to acute and chronic water loading. Compared with wild-type (+/+) controls, mPGES-1 -/- mice exhibited impaired ability to excrete an acute, but not chronic water load. In response to acute water loading, urinary PGE2 excretion in the +/+ mice increased at 2 h, in parallel with increased urine flow. In contrast, the -/- mice exhibited a delayed increase in urinary PGE2 excretion, coinciding with the stimulation of renal medullary mRNA expression of cytosolic prostaglandin E synthase but not mPGES-2. At baseline, renal aquaporin-2 (AQP2) expression in mPGES-1 -/- mice was enhanced compared with the +/+ control. In response to acute water loading, renal AQP2 expression in the +/+ mice was significantly reduced, and this reduction was blunted in the -/- mice. Despite striking changes in AQP2 protein expression, renal AQP2 mRNA in both genotypes largely remained unchanged. Overall, these data support an important role of mPGES-1 in provoking the diuretic response to acute water loading.
AB - PGE2 has an established role in renal water handling. The present study was undertaken to examine the role of microsomal prostaglandin E synthase-1 (mPGES-1) in the diuretic response to acute and chronic water loading. Compared with wild-type (+/+) controls, mPGES-1 -/- mice exhibited impaired ability to excrete an acute, but not chronic water load. In response to acute water loading, urinary PGE2 excretion in the +/+ mice increased at 2 h, in parallel with increased urine flow. In contrast, the -/- mice exhibited a delayed increase in urinary PGE2 excretion, coinciding with the stimulation of renal medullary mRNA expression of cytosolic prostaglandin E synthase but not mPGES-2. At baseline, renal aquaporin-2 (AQP2) expression in mPGES-1 -/- mice was enhanced compared with the +/+ control. In response to acute water loading, renal AQP2 expression in the +/+ mice was significantly reduced, and this reduction was blunted in the -/- mice. Despite striking changes in AQP2 protein expression, renal AQP2 mRNA in both genotypes largely remained unchanged. Overall, these data support an important role of mPGES-1 in provoking the diuretic response to acute water loading.
KW - Aquaporin-2
KW - Collecting duct
KW - Diuresis
KW - Water loading
UR - http://www.scopus.com/inward/record.url?scp=66049156636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66049156636&partnerID=8YFLogxK
U2 - 10.1152/ajprenal.90478.2008
DO - 10.1152/ajprenal.90478.2008
M3 - Article
C2 - 19225050
AN - SCOPUS:66049156636
VL - 296
SP - F1129-F1135
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 5
ER -