mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression

Nathalie Sans, Philip Y. Wang, Quansheng Du, Ronald S. Petralia, Ya Xian Wang, Sajan Nakka, Joe B. Blumer, Ian G. Macara, Robert J. Wenthold

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Appropriate trafficking and targeting of glutamate receptors (GluRs) to the postsynaptic density is crucial for synaptic function. We show that mPins (mammalian homologue of Drosophila melanogaster partner of inscuteable) interacts with SAP102 and PSD-95 (two PDZ proteins present in neurons), and functions in the formation of the NMDAR -MAGUK (N-methyl-D-aspartate receptor -membrane-associated guanylate kinase) complex. mPins enhances trafficking of SAP102 and NMDARs to the plasma membrane in neurons. Expression of dominant -negative constructs and short-interfering RNA (siRNA)-mediated knockdown of mPins decreases SAP102 in dendrites and modifies surface expression of NMDARs. mPins changes the number and morphology of dendritic spines and these effects depend on its Gαi interaction domain, thus implicating G-protein signalling in the regulation of postsynaptic structure and trafficking of GluRs.

Original languageEnglish (US)
Pages (from-to)1079-1090
Number of pages12
JournalNature Cell Biology
Volume7
Issue number12
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

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Glutamate Receptors
N-Methyl-D-Aspartate Receptors
Guanylate Kinases
Post-Synaptic Density
Neurons
Dendritic Spines
Dendrites
Drosophila melanogaster
GTP-Binding Proteins
Small Interfering RNA
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sans, N., Wang, P. Y., Du, Q., Petralia, R. S., Wang, Y. X., Nakka, S., ... Wenthold, R. J. (2005). mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression. Nature Cell Biology, 7(12), 1079-1090. https://doi.org/10.1038/ncb1325

mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression. / Sans, Nathalie; Wang, Philip Y.; Du, Quansheng; Petralia, Ronald S.; Wang, Ya Xian; Nakka, Sajan; Blumer, Joe B.; Macara, Ian G.; Wenthold, Robert J.

In: Nature Cell Biology, Vol. 7, No. 12, 01.12.2005, p. 1079-1090.

Research output: Contribution to journalArticle

Sans, N, Wang, PY, Du, Q, Petralia, RS, Wang, YX, Nakka, S, Blumer, JB, Macara, IG & Wenthold, RJ 2005, 'mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression', Nature Cell Biology, vol. 7, no. 12, pp. 1079-1090. https://doi.org/10.1038/ncb1325
Sans, Nathalie ; Wang, Philip Y. ; Du, Quansheng ; Petralia, Ronald S. ; Wang, Ya Xian ; Nakka, Sajan ; Blumer, Joe B. ; Macara, Ian G. ; Wenthold, Robert J. / mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression. In: Nature Cell Biology. 2005 ; Vol. 7, No. 12. pp. 1079-1090.
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