mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression

Nathalie Sans, Philip Y. Wang, Quansheng Du, Ronald S. Petralia, Ya Xian Wang, Sajan Nakka, Joe B. Blumer, Ian G. Macara, Robert J. Wenthold

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Appropriate trafficking and targeting of glutamate receptors (GluRs) to the postsynaptic density is crucial for synaptic function. We show that mPins (mammalian homologue of Drosophila melanogaster partner of inscuteable) interacts with SAP102 and PSD-95 (two PDZ proteins present in neurons), and functions in the formation of the NMDAR -MAGUK (N-methyl-D-aspartate receptor -membrane-associated guanylate kinase) complex. mPins enhances trafficking of SAP102 and NMDARs to the plasma membrane in neurons. Expression of dominant -negative constructs and short-interfering RNA (siRNA)-mediated knockdown of mPins decreases SAP102 in dendrites and modifies surface expression of NMDARs. mPins changes the number and morphology of dendritic spines and these effects depend on its Gαi interaction domain, thus implicating G-protein signalling in the regulation of postsynaptic structure and trafficking of GluRs.

Original languageEnglish (US)
Pages (from-to)1079-1090
Number of pages12
JournalNature Cell Biology
Volume7
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'mPins modulates PSD-95 and SAP102 trafficking and influences NMDA receptor surface expression'. Together they form a unique fingerprint.

Cite this