MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat

Quan Jiang, Christine Thiffault, Brian C. Kramer, Guang Liang Ding, Li Zhang, Siamak P. Nejad-Davarani, Lian Li, Ali Syed Arbab, Mei Lu, Brad Navia, Stephen J. Victor, Klaudyne Hong, Qing Jiang Li, Shi Yang Wang, Yi Li, Michael Chopp

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human umbilical tissue-derived cells (hUTC) represent an attractive cell source and a potential technology for neurorestoration and improvement of functional outcomes following stroke. Male Wistar rats were subjected to a transient middle cerebral artery occlusion (tMCAo) and were intravenously administered hUTC (N = 11) or vehicle (N = 10) 48 hrs after stroke. White matter and vascular reorganization was monitored over a 12-week period using MRI and histopathology. MRI results were correlated with neurological functional and histology outcomes to demonstrate that MRI can be a useful tool to measure structural recovery after stroke. MRI revealed a significant reduction in the ventricular volume expansion and improvement in cerebral blood flow (CBF) in the hUTC treated group compared to vehicle treated group. Treatment with hUTC resulted in histological and functional improvements as evidenced by enhanced expression of vWF and synaptophysin, and improved outcomes on behavioral tests. Significant correlations were detected between MRI ventricular volumes and histological lesion volume as well as number of apoptotic cells. A positive correlation was also observed between MRI CBF or cerebral blood volume (CBV) and histological synaptic density. Neurological functional tests were also significantly correlated with MRI ventricular volume and CBV. Our data demonstrated that MRI measurements can detect the effect of hUTC therapy on the brain reorganization and exhibited positive correlation with histological measurements of brain structural changes and functional behavioral tests after stroke. MRI ventricular volumes provided the most sensitive index in monitoring brain remodeling and treatment effects and highly correlated with histological and functional measurements.

Original languageEnglish (US)
Article numbere42845
JournalPLoS One
Volume7
Issue number8
DOIs
StatePublished - Aug 10 2012
Externally publishedYes

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umbilicus
Umbilicus
stroke
Magnetic resonance imaging
Rats
Brain
Stroke
Tissue
Cerebrovascular Circulation
brain
rats
Blood
cells
blood volume
blood flow
Therapeutics
Synaptophysin
Middle Cerebral Artery Infarction
Cell- and Tissue-Based Therapy
Blood Vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Jiang, Q., Thiffault, C., Kramer, B. C., Ding, G. L., Zhang, L., Nejad-Davarani, S. P., ... Chopp, M. (2012). MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat. PLoS One, 7(8), [e42845]. https://doi.org/10.1371/journal.pone.0042845

MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat. / Jiang, Quan; Thiffault, Christine; Kramer, Brian C.; Ding, Guang Liang; Zhang, Li; Nejad-Davarani, Siamak P.; Li, Lian; Arbab, Ali Syed; Lu, Mei; Navia, Brad; Victor, Stephen J.; Hong, Klaudyne; Li, Qing Jiang; Wang, Shi Yang; Li, Yi; Chopp, Michael.

In: PLoS One, Vol. 7, No. 8, e42845, 10.08.2012.

Research output: Contribution to journalArticle

Jiang, Q, Thiffault, C, Kramer, BC, Ding, GL, Zhang, L, Nejad-Davarani, SP, Li, L, Arbab, AS, Lu, M, Navia, B, Victor, SJ, Hong, K, Li, QJ, Wang, SY, Li, Y & Chopp, M 2012, 'MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat', PLoS One, vol. 7, no. 8, e42845. https://doi.org/10.1371/journal.pone.0042845
Jiang, Quan ; Thiffault, Christine ; Kramer, Brian C. ; Ding, Guang Liang ; Zhang, Li ; Nejad-Davarani, Siamak P. ; Li, Lian ; Arbab, Ali Syed ; Lu, Mei ; Navia, Brad ; Victor, Stephen J. ; Hong, Klaudyne ; Li, Qing Jiang ; Wang, Shi Yang ; Li, Yi ; Chopp, Michael. / MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat. In: PLoS One. 2012 ; Vol. 7, No. 8.
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