MRI detects white matter reorganization after neural progenitor cell treatment of stroke

Quan Jiang, Zheng Gang Zhang, Guang Liang Ding, Brian Silver, Li Zhang, He Meng, Mei Lu, Siamak Pourabdillah-Nejed-D., Lei Wang, Smita Savant-Bhonsale, Lian Li, Hassan Bagher-Ebadian, Jiani Hu, Ali Syed Arbab, Padmavathy Vanguri, James R. Ewing, Karyn Alayne Ledbetter, Michael Chopp

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.

Original languageEnglish (US)
Pages (from-to)1080-1089
Number of pages10
JournalNeuroImage
Volume32
Issue number3
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

Fingerprint

Stem Cells
Stroke
Therapeutics
Diffusion Tensor Imaging
Corpus Callosum
Middle Cerebral Artery Infarction
Anisotropy
Wistar Rats
White Matter
Staining and Labeling
Neurons
Control Groups

Keywords

  • Cerebral ischemia
  • Diffusion tensor imaging
  • Magnetic resonance imaging
  • Molecular imaging
  • Neuroplasticity
  • White matter reorganization

ASJC Scopus subject areas

  • Neurology
  • Cognitive Neuroscience

Cite this

Jiang, Q., Zhang, Z. G., Ding, G. L., Silver, B., Zhang, L., Meng, H., ... Chopp, M. (2006). MRI detects white matter reorganization after neural progenitor cell treatment of stroke. NeuroImage, 32(3), 1080-1089. https://doi.org/10.1016/j.neuroimage.2006.05.025

MRI detects white matter reorganization after neural progenitor cell treatment of stroke. / Jiang, Quan; Zhang, Zheng Gang; Ding, Guang Liang; Silver, Brian; Zhang, Li; Meng, He; Lu, Mei; Pourabdillah-Nejed-D., Siamak; Wang, Lei; Savant-Bhonsale, Smita; Li, Lian; Bagher-Ebadian, Hassan; Hu, Jiani; Arbab, Ali Syed; Vanguri, Padmavathy; Ewing, James R.; Ledbetter, Karyn Alayne; Chopp, Michael.

In: NeuroImage, Vol. 32, No. 3, 01.09.2006, p. 1080-1089.

Research output: Contribution to journalArticle

Jiang, Q, Zhang, ZG, Ding, GL, Silver, B, Zhang, L, Meng, H, Lu, M, Pourabdillah-Nejed-D., S, Wang, L, Savant-Bhonsale, S, Li, L, Bagher-Ebadian, H, Hu, J, Arbab, AS, Vanguri, P, Ewing, JR, Ledbetter, KA & Chopp, M 2006, 'MRI detects white matter reorganization after neural progenitor cell treatment of stroke', NeuroImage, vol. 32, no. 3, pp. 1080-1089. https://doi.org/10.1016/j.neuroimage.2006.05.025
Jiang, Quan ; Zhang, Zheng Gang ; Ding, Guang Liang ; Silver, Brian ; Zhang, Li ; Meng, He ; Lu, Mei ; Pourabdillah-Nejed-D., Siamak ; Wang, Lei ; Savant-Bhonsale, Smita ; Li, Lian ; Bagher-Ebadian, Hassan ; Hu, Jiani ; Arbab, Ali Syed ; Vanguri, Padmavathy ; Ewing, James R. ; Ledbetter, Karyn Alayne ; Chopp, Michael. / MRI detects white matter reorganization after neural progenitor cell treatment of stroke. In: NeuroImage. 2006 ; Vol. 32, No. 3. pp. 1080-1089.
@article{53b016c5fa3b44d1abb30938ceee9978,
title = "MRI detects white matter reorganization after neural progenitor cell treatment of stroke",
abstract = "We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.",
keywords = "Cerebral ischemia, Diffusion tensor imaging, Magnetic resonance imaging, Molecular imaging, Neuroplasticity, White matter reorganization",
author = "Quan Jiang and Zhang, {Zheng Gang} and Ding, {Guang Liang} and Brian Silver and Li Zhang and He Meng and Mei Lu and Siamak Pourabdillah-Nejed-D. and Lei Wang and Smita Savant-Bhonsale and Lian Li and Hassan Bagher-Ebadian and Jiani Hu and Arbab, {Ali Syed} and Padmavathy Vanguri and Ewing, {James R.} and Ledbetter, {Karyn Alayne} and Michael Chopp",
year = "2006",
month = "9",
day = "1",
doi = "10.1016/j.neuroimage.2006.05.025",
language = "English (US)",
volume = "32",
pages = "1080--1089",
journal = "NeuroImage",
issn = "1053-8119",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - MRI detects white matter reorganization after neural progenitor cell treatment of stroke

AU - Jiang, Quan

AU - Zhang, Zheng Gang

AU - Ding, Guang Liang

AU - Silver, Brian

AU - Zhang, Li

AU - Meng, He

AU - Lu, Mei

AU - Pourabdillah-Nejed-D., Siamak

AU - Wang, Lei

AU - Savant-Bhonsale, Smita

AU - Li, Lian

AU - Bagher-Ebadian, Hassan

AU - Hu, Jiani

AU - Arbab, Ali Syed

AU - Vanguri, Padmavathy

AU - Ewing, James R.

AU - Ledbetter, Karyn Alayne

AU - Chopp, Michael

PY - 2006/9/1

Y1 - 2006/9/1

N2 - We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.

AB - We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.

KW - Cerebral ischemia

KW - Diffusion tensor imaging

KW - Magnetic resonance imaging

KW - Molecular imaging

KW - Neuroplasticity

KW - White matter reorganization

UR - http://www.scopus.com/inward/record.url?scp=33747806913&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747806913&partnerID=8YFLogxK

U2 - 10.1016/j.neuroimage.2006.05.025

DO - 10.1016/j.neuroimage.2006.05.025

M3 - Article

VL - 32

SP - 1080

EP - 1089

JO - NeuroImage

JF - NeuroImage

SN - 1053-8119

IS - 3

ER -