TY - JOUR
T1 - MRI detects white matter reorganization after neural progenitor cell treatment of stroke
AU - Jiang, Quan
AU - Zhang, Zheng Gang
AU - Ding, Guang Liang
AU - Silver, Brian
AU - Zhang, Li
AU - Meng, He
AU - Lu, Mei
AU - Pourabdillah-Nejed-D., Siamak
AU - Wang, Lei
AU - Savant-Bhonsale, Smita
AU - Li, Lian
AU - Bagher-Ebadian, Hassan
AU - Hu, Jiani
AU - Arbab, Ali Syed
AU - Vanguri, Padmavathy
AU - Ewing, James R.
AU - Ledbetter, Karyn Alayne
AU - Chopp, Michael
N1 - Funding Information:
We thank Dr. Susumu Mori for providing the software DtiStadio to perform DTI data analysis, Qing Jiang Li, Polly Whitton, and Hemanthkumar Athiraman for data analysis, and Swayam Panda for data acquisitions. This work was supported by NIH grants RO1 NS48349, RO1 NS38292, RO1 NS43324, RO1 HL64766, PO1 NS23393, and PO1 NS42345.
PY - 2006/9
Y1 - 2006/9
N2 - We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.
AB - We evaluated the effects of neural progenitor cell treatment of stroke on white matter reorganization using MRI. Male Wistar rats (n = 26) were subjected to 3 h of middle cerebral artery occlusion and were treated with neural progenitor cells (n = 17) or without treatment (n = 9) and were sacrificed at 5-7 weeks thereafter. MRI measurements revealed that grafted neural progenitor cells selectively migrated towards the ischemic boundary regions. White matter reorganization, confirmed histologically, was coincident with increases of fractional anisotropy (FA, P < 0.01) after stroke in the ischemic recovery regions compared to that in the ischemic core region in both treated and control groups. Immunoreactive staining showed axonal projections emanating from neurons and extruding from the corpus callosum into the ipsilateral striatum bounding the lesion areas after stroke. Fiber tracking (FT) maps derived from diffusion tensor imaging revealed similar orientation patterns to the immunohistological results. Complementary measurements in stroke patients indicated that FT maps exhibit an overall orientation parallel to the lesion boundary. Our data demonstrate that FA and FT identify and characterize cerebral tissue undergoing white matter reorganization after stroke and treatment with neural progenitor cells.
KW - Cerebral ischemia
KW - Diffusion tensor imaging
KW - Magnetic resonance imaging
KW - Molecular imaging
KW - Neuroplasticity
KW - White matter reorganization
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U2 - 10.1016/j.neuroimage.2006.05.025
DO - 10.1016/j.neuroimage.2006.05.025
M3 - Article
C2 - 16860575
AN - SCOPUS:33747806913
SN - 1053-8119
VL - 32
SP - 1080
EP - 1089
JO - NeuroImage
JF - NeuroImage
IS - 3
ER -