TY - JOUR
T1 - mRNA for the three human alcohol dehydrogenase subunits
T2 - Size heterogeneity and developmental changes
AU - Ikuta, T.
AU - Yoshida, A.
N1 - Funding Information:
*This work was supported by NIH grant AAOS763. ABBREVIATIONS: ADH; alcohol dehydrogenase.
PY - 1986/11/14
Y1 - 1986/11/14
N2 - Human alcohol dehydrogenase consists of three types of subunits (α, β, and γ) which are governed by three separate loci. mRNA components for the three subunits were examined by Northern blot hybridization, using a common cDNA probe and specific oligonucleotide probes. A marked size heterogeneity of β mRNA, ranging from 1.6 kb to 5.2 kb (of which the 2.4-kb and 3.5-kb were major), was observed in the adult liver. The mRNA for the γ subunit was homogeneous (about 1.6 kb), and that for the α subunit contained a major 1.6-kb and a minor 4.3-kb component. The amount of mRNA was much lower (∼10% of adult) and the mRNA was less heterogeneous in the infant liver; i.e., an α mRNA (1.6 kb) and two β mRNAs (a major 1.6 kb and a minor 3.5 kb), and no detectable γ mRNA. The short β mRNA, with 1.6 kb, could not be hybridized with a non-coding cDNA fragment that originated from the 3′-end of the 2.6 kb β cDNA. The observed multiple ADH mRNAs, with a common coding region and different 3′-untranslated regions, are generated by utilization of multiple polyadenylation signals. The selection of polyadenylation signals and the rate of transcription of the three ADH loci are developmentally regulated. The 4.3-kb-long mRNA, which was hybridized with both α- and β-specific probes, could be related to the previously cloned "fused β-α" cDNA.
AB - Human alcohol dehydrogenase consists of three types of subunits (α, β, and γ) which are governed by three separate loci. mRNA components for the three subunits were examined by Northern blot hybridization, using a common cDNA probe and specific oligonucleotide probes. A marked size heterogeneity of β mRNA, ranging from 1.6 kb to 5.2 kb (of which the 2.4-kb and 3.5-kb were major), was observed in the adult liver. The mRNA for the γ subunit was homogeneous (about 1.6 kb), and that for the α subunit contained a major 1.6-kb and a minor 4.3-kb component. The amount of mRNA was much lower (∼10% of adult) and the mRNA was less heterogeneous in the infant liver; i.e., an α mRNA (1.6 kb) and two β mRNAs (a major 1.6 kb and a minor 3.5 kb), and no detectable γ mRNA. The short β mRNA, with 1.6 kb, could not be hybridized with a non-coding cDNA fragment that originated from the 3′-end of the 2.6 kb β cDNA. The observed multiple ADH mRNAs, with a common coding region and different 3′-untranslated regions, are generated by utilization of multiple polyadenylation signals. The selection of polyadenylation signals and the rate of transcription of the three ADH loci are developmentally regulated. The 4.3-kb-long mRNA, which was hybridized with both α- and β-specific probes, could be related to the previously cloned "fused β-α" cDNA.
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U2 - 10.1016/0006-291X(86)90737-0
DO - 10.1016/0006-291X(86)90737-0
M3 - Article
C2 - 2877665
AN - SCOPUS:0022977728
SN - 0006-291X
VL - 140
SP - 1020
EP - 1027
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -