Multiple biological activities of human recombinant interleukin 1

C. A. Dinarello, Joseph Gerard Cannon, J. W. Mier, H. A. Bernheim, G. LoPreste, D. L. Lynn, R. N. Love, A. C. Webb, P. E. Auron, R. C. Reuben

Research output: Contribution to journalArticle

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Abstract

Complementary DNA coding for human monocyte interleukin 1 (IL-1), pI 7 form, was expressed in Escherichia coli. During purification, IL-1 activity on murine T cells was associated with the recombinant protein. Homogeneous human recombinant IL-1 (hrIL-1) was tested in several assays to demonstrate the immunological and inflammatory properties attributed to this molecule. hrIL-1 induced proliferative responses in a cloned murine T cell in the presence of suboptimal concentrations of mitogen, whereas no effect was observed with hrIL-1 alone. At concentrations of 0.05 ng/ml, hrIL-1 doubled the response to mitogen (5 x 106 half maximal units/mg). Human peripheral blood T cells depleted of adherent cells underwent a blastogenic response and released interleukin 2 in the presence of hrIL-1 and mitogen. hrIL-1 was a potent inflammatory agent by its ability to induce human dermal fibroblast prostaglandin E2 production in vitro and to produce monophasic (endogenous pyrogen) fever when injected into rabbits or endotoxin-resistant mice. These studies establish that the dominant pI 7 form of recombinant human IL-1 possesses immunological and inflammatory properties and acts on the central nervous system to produce fever.

Original languageEnglish (US)
Pages (from-to)1734-1739
Number of pages6
JournalJournal of Clinical Investigation
Volume77
Issue number6
DOIs
StatePublished - Jan 1 1986

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Interleukin-1
Human Activities
Mitogens
T-Lymphocytes
Fever
Recombinant Proteins
Dinoprostone
Endotoxins
Interleukin-2
Monocytes
Blood Cells
Central Nervous System
Complementary DNA
Fibroblasts
Escherichia coli
Rabbits
Skin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Dinarello, C. A., Cannon, J. G., Mier, J. W., Bernheim, H. A., LoPreste, G., Lynn, D. L., ... Reuben, R. C. (1986). Multiple biological activities of human recombinant interleukin 1. Journal of Clinical Investigation, 77(6), 1734-1739. https://doi.org/10.1172/JCI112495

Multiple biological activities of human recombinant interleukin 1. / Dinarello, C. A.; Cannon, Joseph Gerard; Mier, J. W.; Bernheim, H. A.; LoPreste, G.; Lynn, D. L.; Love, R. N.; Webb, A. C.; Auron, P. E.; Reuben, R. C.

In: Journal of Clinical Investigation, Vol. 77, No. 6, 01.01.1986, p. 1734-1739.

Research output: Contribution to journalArticle

Dinarello, CA, Cannon, JG, Mier, JW, Bernheim, HA, LoPreste, G, Lynn, DL, Love, RN, Webb, AC, Auron, PE & Reuben, RC 1986, 'Multiple biological activities of human recombinant interleukin 1', Journal of Clinical Investigation, vol. 77, no. 6, pp. 1734-1739. https://doi.org/10.1172/JCI112495
Dinarello CA, Cannon JG, Mier JW, Bernheim HA, LoPreste G, Lynn DL et al. Multiple biological activities of human recombinant interleukin 1. Journal of Clinical Investigation. 1986 Jan 1;77(6):1734-1739. https://doi.org/10.1172/JCI112495
Dinarello, C. A. ; Cannon, Joseph Gerard ; Mier, J. W. ; Bernheim, H. A. ; LoPreste, G. ; Lynn, D. L. ; Love, R. N. ; Webb, A. C. ; Auron, P. E. ; Reuben, R. C. / Multiple biological activities of human recombinant interleukin 1. In: Journal of Clinical Investigation. 1986 ; Vol. 77, No. 6. pp. 1734-1739.
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