Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

A. E. Ferry, Betty Sue Pace

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Obtaining adequate samples for nucleic acid or protein analysis from a limited number of cells can be a difficult task. The steps for isolation of DNA, cytoplasmic RNA and nuclear proteins from mononuclear cells collected from a single peripheral blood sample are outlined below. A previously described technique for rapid isolation of nuclear proteins was modified to acquire RNA and DNA of sufficient quantity and quality to perform analyses at the molecular level without altering the quality of protein extracted. This approach is applicable for use with peripheral blood mononuclear cells, primary cultures and immortalized cell lines.

Original languageEnglish (US)
Pages (from-to)375-378
Number of pages4
JournalCellular and Molecular Biology Letters
Volume3
Issue number4
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Nuclear Proteins
RNA
Blood
DNA
Cell culture
Nucleic Acids
Blood Cells
Proteins
Cell Culture Techniques
Cell Count
Cells
Cell Line

Keywords

  • DNA Isolation
  • Mononuclear Cells
  • Protein Extraction
  • RNA Isolation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood. / Ferry, A. E.; Pace, Betty Sue.

In: Cellular and Molecular Biology Letters, Vol. 3, No. 4, 01.01.1998, p. 375-378.

Research output: Contribution to journalArticle

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