Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins

Sharad B Purohit, Tiehai Li, Wanyi Guan, Xuezheng Song, Jing Song, Yanna Tian, Lei Li, Ashok Kumar Sharma, Boying Dun, David Mysona, Sharad A Ghamande, Bunja Jane Rungruang, Richard D. Cummings, Peng George Wang, Jin-Xiong She

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glycan-binding proteins (GBPs) play critical roles in diverse cellular functions such as cell adhesion, signal transduction and immune response. Studies of the interaction between GBPs and glycans have been hampered by the availability of high throughput and high-content technologies. Here we report multiplex glycan bead array (MGBA) that allows simultaneous analyses of 384 samples and up to 500 glycans in a single assay. The specificity, sensitivity and reproducibility of MGBA are evaluated using 39 plant lectins, 13 recombinant anti-glycan antibodies, and mammalian GBPs. We demonstrate the utility of this platform by the analyses of natural anti-glycan IgM and IgG antibodies in 961 human serum samples and the discovery of anti-glycan antibody biomarkers for ovarian cancer. Our data indicate that the MGBA platform is particularly suited for large population-based studies that require the analyses of large numbers of samples and glycans.

Original languageEnglish (US)
Article number258
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

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antibodies
beads
Polysaccharides
Carrier Proteins
Throughput
proteins
platforms
biomarkers
serums
availability
adhesion
cancer
sensitivity
cells
Antibodies
Anti-Idiotypic Antibodies
interactions
Plant Lectins
Signal transduction
Cell adhesion

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. / Purohit, Sharad B; Li, Tiehai; Guan, Wanyi; Song, Xuezheng; Song, Jing; Tian, Yanna; Li, Lei; Sharma, Ashok Kumar; Dun, Boying; Mysona, David; Ghamande, Sharad A; Rungruang, Bunja Jane; Cummings, Richard D.; Wang, Peng George; She, Jin-Xiong.

In: Nature Communications, Vol. 9, No. 1, 258, 01.12.2018.

Research output: Contribution to journalArticle

Purohit, Sharad B ; Li, Tiehai ; Guan, Wanyi ; Song, Xuezheng ; Song, Jing ; Tian, Yanna ; Li, Lei ; Sharma, Ashok Kumar ; Dun, Boying ; Mysona, David ; Ghamande, Sharad A ; Rungruang, Bunja Jane ; Cummings, Richard D. ; Wang, Peng George ; She, Jin-Xiong. / Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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AU - Sharma, Ashok Kumar

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