Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies

Sharad Purohit, Jin-Xiong She

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We present here detailed protocols for the newly developed multiplex glycan bead array (MGBA) for the high throughput and high content analyses of various glycan-binding proteins including anti-glycan antibodies. This platform takes advantage of the commercially available Luminex beads to construct glycan arrays that are easily customizable at will and anytime by researchers. The platform allows the simultaneous analyses of up to 500 glycans and 384 samples at a time. By using multiple arrays, a researcher can analyze thousands of glycans and tens of thousands of samples within a short period. The assay is highly sensitive, specific, reproducible, economic, and fast. Furthermore, the bead array platform is approved for use in clinical settings, speeding up the translation of laboratory discoveries into patient care.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-44
Number of pages12
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2460
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Arrays
  • Beads
  • Biomarkers
  • Carbohydrates
  • Glycan antibodies
  • Glycans
  • Glycoconjugates
  • Luminex
  • Polysaccharides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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