TY - JOUR
T1 - Murine α-l-Iduronidase
T2 - cDNA isolation and expression
AU - Clarke, Lorne A.
AU - Nasir, Jamal
AU - Zhang, Hanfang
AU - McDonald, Helen
AU - Applegarth, Derek A.
AU - Hayden, Michael R.
AU - Toone, Jennifer
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994/11/15
Y1 - 1994/11/15
N2 - As an initial step toward the generation of a murine model for mucopolysaccharidosis type I, we have identified and characterized a full-length murine α-l-iduronidase cDNA. Expression of the murine cDNA in COS-1 cells results in the production of α-l-iduronidase enzyme activity at a level 20-fold higher than that of the endogenous gene. The murine cDNA shows strong homology with the coding region of both the human and the canine homologs with 78 and 75% nucleotide sequence identity, respectively. In contrast to the coding region, significant diversity of sequence exists for the 5′ and 3′ untranslated regions between the murine and both the human and the canine sequence. The 3′ UTR of the murine transcript is 1193 bp in length, as compared to the human (100 bp) and canine (139 bp), and contains a CA dinucleotide repeat not seen in either the human or the canine genes. A portion of the murine iduronidase coding sequence overlaps with sequence reported for the 3′ UTR of the murine SAT-1 cDNA. The sequence overlap involves the proposed exon II of murine iduronidase and covers 141 bp of sequence with the transcripts generated in opposite orientation. We report here the characterization of murine α-l-iduronidase cDNA and its relationship to SAT-1.
AB - As an initial step toward the generation of a murine model for mucopolysaccharidosis type I, we have identified and characterized a full-length murine α-l-iduronidase cDNA. Expression of the murine cDNA in COS-1 cells results in the production of α-l-iduronidase enzyme activity at a level 20-fold higher than that of the endogenous gene. The murine cDNA shows strong homology with the coding region of both the human and the canine homologs with 78 and 75% nucleotide sequence identity, respectively. In contrast to the coding region, significant diversity of sequence exists for the 5′ and 3′ untranslated regions between the murine and both the human and the canine sequence. The 3′ UTR of the murine transcript is 1193 bp in length, as compared to the human (100 bp) and canine (139 bp), and contains a CA dinucleotide repeat not seen in either the human or the canine genes. A portion of the murine iduronidase coding sequence overlaps with sequence reported for the 3′ UTR of the murine SAT-1 cDNA. The sequence overlap involves the proposed exon II of murine iduronidase and covers 141 bp of sequence with the transcripts generated in opposite orientation. We report here the characterization of murine α-l-iduronidase cDNA and its relationship to SAT-1.
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U2 - 10.1006/geno.1994.1621
DO - 10.1006/geno.1994.1621
M3 - Article
C2 - 7698753
AN - SCOPUS:0028670604
SN - 0888-7543
VL - 24
SP - 311
EP - 316
JO - Genomics
JF - Genomics
IS - 2
ER -