Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion

Angelica Belo, Kunrong Cheng, Ahmed Chahdi, Jasleen Shant, Guofeng Xie, Sandeep Khurana, Jean Pierre Raufman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Muscarinic receptors (CHRM) are overexpressed in colon cancer. To explore a role for muscarinic receptor signaling in colon cancer metastasis, we used human H508 and HT29 colon cancer cells that coexpress epidermal growth factor (ERBB) and CHRM3 receptors. In a wound closure model, following 8-h incubation of H508 cells with 100 μM ACh we observed a threefold increase in cell migration indistinguishable from the actions of epidermal growth factor (EGF). Atropine blocked the actions of ACh but not of EGF. In SNU-C4 colon cancer cells that express ERBB but not CHRM, EGF caused a threefold increase in migration; ACh had no effect. ACh-induced cell migration was attenuated by chemical inhibitors of ERBB1 activation, by anti-ERBB1 antibody, and by inhibitors of ERK and phosphatidylinositol 3-kinase (PI3K) signaling. Consistent with matrix metalloproteinase-7 (MMP7)-mediated release of an ERBB1 ligand, heparin binding epidermal growth factor-like growth factor (HBEGF), ACh-induced migration was inhibited by an MMP inhibitor and by anti-MMP7 and -HBEGF antibodies. AChinduced cell migration was blocked by inhibiting RhoA and ROCK, key proteins that interact with the actin cytoskeleton. ACh-induced RhoA activation was attenuated by agents that inhibit ERBB1, ERK, and PI3K activation. Collectively, these findings indicate that AChinduced cell migration is mediated by MMP7-mediated release of HBEGF, an ERBB ligand that activates ERBB1 and downstream ERK and PI3K signaling. In a cell invasion model, ACh-induced HT29 cell invasion was blocked by atropine. In concert with previous observations, these findings indicate that muscarinic receptor signaling plays a key role in colon cancer cell proliferation, survival, migration, and invasion.

Original languageEnglish (US)
Pages (from-to)G749-G760
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume300
Issue number5
DOIs
StatePublished - May 1 2011

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Muscarinic Agonists
Muscarinic Receptors
Epidermal Growth Factor
Colonic Neoplasms
Cell Movement
Phosphatidylinositol 3-Kinase
Matrix Metalloproteinase 7
Heparin
Intercellular Signaling Peptides and Proteins
Atropine
Ligands
HT29 Cells
Matrix Metalloproteinase Inhibitors
Actin Cytoskeleton
Anti-Idiotypic Antibodies
Cell Survival
Cell Proliferation
Neoplasm Metastasis
Antibodies
Wounds and Injuries

Keywords

  • Acetylcholine
  • Actin cytoskeleton
  • Mitogen-activated protein kinase
  • Muscarinic receptors
  • Tumor metastasis

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion. / Belo, Angelica; Cheng, Kunrong; Chahdi, Ahmed; Shant, Jasleen; Xie, Guofeng; Khurana, Sandeep; Raufman, Jean Pierre.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 300, No. 5, 01.05.2011, p. G749-G760.

Research output: Contribution to journalArticle

Belo, Angelica ; Cheng, Kunrong ; Chahdi, Ahmed ; Shant, Jasleen ; Xie, Guofeng ; Khurana, Sandeep ; Raufman, Jean Pierre. / Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2011 ; Vol. 300, No. 5. pp. G749-G760.
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T1 - Muscarinic receptor agonists stimulate human colon cancer cell migration and invasion

AU - Belo, Angelica

AU - Cheng, Kunrong

AU - Chahdi, Ahmed

AU - Shant, Jasleen

AU - Xie, Guofeng

AU - Khurana, Sandeep

AU - Raufman, Jean Pierre

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