Muscarinic receptor-mediated hydrolysis of phosphatidylinositols in human neuroblastoma (SH-SY5Y) cells is sensitive to pertussis toxin

Lin Mei, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/106 cells to 42.4 fmol/106 cells (P < 0.05) and 22.1 fmol/106 cells (P < 0.01) in the absence and presence of 1 μg/ml and 10 μg/ml pertussis toxin, respectively while the EC50 values did not change. Cholera toxin (1 mg/ml) did not alter ca carbachol-stimulated [3H]IP1 accumulation in these cells. These results suggests that a pertussis toxin sensitive G-protein may be involved in muscarinic receptor-phosphatidylinositol hydrolysis coupling in SH-SY5Y cells.

Original languageEnglish (US)
Pages (from-to)360-363
Number of pages4
JournalBrain Research
Volume447
Issue number2
DOIs
StatePublished - May 3 1988

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Keywords

  • Human neuroblastoma
  • Muscarinic receptor
  • Pertussis toxin
  • Phosphatidylinositol hydrolysis
  • SH-SY5Y cell line

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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