Mutagenesis and characterization of specific residues in fatty acid ethyl ester synthase

A gene for alcohol-induced cardiomyopathy

Puran S. Bora, Donald D Miller, Bernard R. Chaitman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fatty acid ethyl ester synthase-III metabolizes both ethanol and carcinogens, Structure-function studies of the enzyme have not been performed in relation to site specific mutagenesis. In this study, three residues (Gly 32, Cys 39 and His 72) have been mutated to observe their role in enzyme activity. Gly to Gln, Cys to Trp and His to Ser mutations did not affect fatty acid ethyl ester synthase activity, but His to Ser mutant had less than 9% of control glutathione S-transferase activity. The apparent loss of transferase activity reflected a 28 fold weaker binding constant for glutathione. Thus, this study indicates that Gly and Cys may not be important for synthase or transferase activities however, histidine may play a role in glutathione binding, but it is not an essential catalytic residue of glutathione S-transferase or for fatty acid ethyl ester synthase activity.

Original languageEnglish (US)
Pages (from-to)111-115
Number of pages5
JournalMolecular and Cellular Biochemistry
Volume180
Issue number1-2
DOIs
StatePublished - Mar 28 1998

Fingerprint

Mutagenesis
Transferases
Glutathione Transferase
Cardiomyopathies
Glutathione
Genes
Alcohols
Enzyme activity
Enzymes
Site-Directed Mutagenesis
Histidine
Carcinogens
Ethanol
Mutation
fatty acyl ethyl ester synthase
human GSTP1 protein

Keywords

  • Carcinogens
  • Cardiomyopathy
  • Catalysis
  • Enzymes
  • Ethanol
  • Mutations

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Mutagenesis and characterization of specific residues in fatty acid ethyl ester synthase : A gene for alcohol-induced cardiomyopathy. / Bora, Puran S.; Miller, Donald D; Chaitman, Bernard R.

In: Molecular and Cellular Biochemistry, Vol. 180, No. 1-2, 28.03.1998, p. 111-115.

Research output: Contribution to journalArticle

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