Abstract
8-Nitroguanosine is a nitratively modified nucleoside that is formed endogeneously under inflammatory conditions dependent on nitric oxide production, particularly associated with cancer risks. Here, we investigated the mutagenic potential of 8-nitroguanosine in mammalian cells. Treatment with 8-nitroguanosine (10-1000 μM) for 1 h significantly increased (by 6-8 times) the mutation frequency of the xanthine-guanine phosphoribosyltransferase (gpt) gene in AS52 cells without cytotoxic effects. 8-Nitroguanosine treatment induced a G-to-T transversion in gpt gene at position 86. It also significantly increased levels of abasic sites in DNA. These observations suggest that formation of 8-nitroguanosine may contribute to the pathogenesis of inflammation-associated carcinogenesis.
Original language | English (US) |
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Pages (from-to) | 239-247 |
Number of pages | 9 |
Journal | Cancer Letters |
Volume | 262 |
Issue number | 2 |
DOIs | |
State | Published - Apr 18 2008 |
Externally published | Yes |
Keywords
- 8-Nitroguanosine
- Abasic site
- Inflammation
- Mutation
- Nitric oxide
ASJC Scopus subject areas
- Oncology
- Cancer Research