Mutant p53 in cell adhesion and motility

W. Andrew Yeudall, Katharine H. Wrighton, Sumitra Deb

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

Original languageEnglish (US)
Title of host publicationp53 Protocols
EditorsSumitra Deb, Swati Palit Deb
Pages135-146
Number of pages12
DOIs
StatePublished - Jan 8 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume962
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Adhesion
  • Anchorage independence
  • Chemotaxis
  • Extracellular matrix
  • Gain-of-function
  • Migration
  • Motility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yeudall, W. A., Wrighton, K. H., & Deb, S. (2013). Mutant p53 in cell adhesion and motility. In S. Deb, & S. P. Deb (Eds.), p53 Protocols (pp. 135-146). (Methods in Molecular Biology; Vol. 962). https://doi.org/10.1007/978-1-62703-236-0-11