Mutant p53 in cell adhesion and motility

W. Andrew Yeudall; Katharine H. Wrighton; Sumitra Deb

doi:10.1007/978-1-62703-236-0_11

Mutant p53 in cell adhesion and motility

W. Andrew Yeudall, Katharine H. Wrighton, Sumitra Deb

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Scopus citations

Abstract

Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

Original language	English (US)
Title of host publication	p53 Protocols
Publisher	Humana Press Inc.
Pages	135-146
Number of pages	12
ISBN (Print)	9781627032353
DOIs	https://doi.org/10.1007/978-1-62703-236-0_11
State	Published - 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	962
ISSN (Print)	1064-3745

Keywords

Adhesion
Anchorage independence
Chemotaxis
Extracellular matrix
Gain-of-function
Migration
Motility

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-236-0_11

Cite this

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title = "Mutant p53 in cell adhesion and motility",

abstract = "Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, {"}wound-healing{"} (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.",

keywords = "Adhesion, Anchorage independence, Chemotaxis, Extracellular matrix, Gain-of-function, Migration, Motility",

author = "Yeudall, {W. Andrew} and Wrighton, {Katharine H.} and Sumitra Deb",

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isbn = "9781627032353",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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T1 - Mutant p53 in cell adhesion and motility

AU - Yeudall, W. Andrew

AU - Wrighton, Katharine H.

AU - Deb, Sumitra

PY - 2013

Y1 - 2013

N2 - Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

AB - Pro-oncogenic properties of mutant p53 were investigated with the aid of migration assays, adhesion assays, and soft agar growth assays using cells stably expressing gain-of-function p53 mutants. To determine cell migration, "wound-healing" (scratch) assays and haptotactic (chamber) assays were used. H1299 cells expressing mutant p53 were found to migrate more rapidly than cells transfected with empty vector alone. Results from both types of migration assay were broadly similar. Migratory ability differed for different p53 mutants, suggesting allele-specific effects. Cells expressing p53 mutants also showed enhanced adhesion to extracellular matrix compare to controls. Furthermore, stable transfection of mutant p53-H179L into NIH3T3 fibroblasts was sufficient to allow anchorage-independent growth in soft agar.

KW - Adhesion

KW - Anchorage independence

KW - Chemotaxis

KW - Extracellular matrix

KW - Gain-of-function

KW - Migration

KW - Motility

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M3 - Chapter

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AN - SCOPUS:84871881742

SN - 9781627032353

T3 - Methods in Molecular Biology

SP - 135

EP - 146

BT - p53 Protocols

PB - Humana Press Inc.

ER -

Mutant p53 in cell adhesion and motility

Abstract

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Keywords

ASJC Scopus subject areas

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