Myelinated dorsal root ganglion cultures activate both the alternative and classical pathways of complement

Patricia Scott-Zaki, Donald Purkall, John Bigbee, Shaun Ruddy, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We used rat myelinated dorsal root ganglion (MDRG) cultures to study antibody and complement-mediated mechanisms of peripheral demyelinating diseases. Heat inactivated serum from a patient (LT) with peripheral neuropathy and a monoclonal IgM reactive with myelin-associated glycoprotein (anti-MAG) and sulfated glucuronosyl glycolipids (anti-SGGL) was used as an antibody source. Incubation of whole human serum (WHS) or WHS and anti-SGGL with MDRGs resulted in reduction of classical and alternative pathway hemolytic activities and the development of abnormal myelin sheaths. Incubation of MDRG cultures with C2-deficient serum showed activation of the alternative complement pathway. Classical pathway hemolytic activity was reduced when Factor B-depleted serum was incubated with MDRG cultures. The rat MDRG culture system provides a good model system of a peripheral nerve and has therefore been used by several investigators to study antibody and complement-mediated demyelination associated with peripheral neuropathies. However, our studies indicate a high degree of complement activation and membrane disruption of cultures incubated with WHS. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)160-169
Number of pages10
JournalJournal of the Neurological Sciences
Volume165
Issue number2
DOIs
StatePublished - Jun 1 1999
Externally publishedYes

Keywords

  • Antibody
  • Complement
  • Demyelination
  • Dorsal root ganglion
  • Glycolipids
  • Rodent

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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