Myocardial function and metabolism in pig hearts after relief from chronic partial coronary stenosis

A. J. Liedtke, B. Renstrom, S. H. Nellis, R. Subramanian

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Metabolic behavior was compared during acute extracorporeal reperfusion after removal of a chronic 4-day partial coronary stenosis in eight pig hearts (RCS group) and during comparable extracorporeal perfusion in seven chronically prepared hearts (Sham group). Coronary stenosis in RCS hearts was induced in the left anterior descending (LAD) artery by partial inflation of a hydraulic occluder to restrict LAD peak phasic velocity by ~50%. Regional mechanical shortening was decreased in RCS compared with Sham hearts after 4 days of chronic coronary stenosis [diminished systolic shortening (P < 0.066) with systolic expansion (P < 0.015)] but was comparable to Sham hearts after relief from stenosis. At analogous workloads (left ventricular pressure and heart rate) during reperfusion, metabolic behavior was distinctive between groups. Specifically, compared with Sham hearts, myocardial O2 consumption was selectively increased in RCS hearts (+49Δ%, P < 0.026) as was fatty acid oxidation estimated from 14CO2 production from [U-14C]palmitate (+60Δ%, P < 0.061) and exogenous glucose utilization measured from the release of 3H2O from [5-3H]glucose (+517Δ%, P < 0.025). At the conclusion of the studies, triphenyltetrazolium chloride staining showed no gross evidence of macroinfarction in RCS or Sham hearts, and there was an essentially unremarkable histological survey of anterior myocardium for microscopic necrosis in either group. The level of O2 consumption and preservation of preferred fatty acid utilization indicate that metabolism remains or regains its aerobic pattern of activity in early recovery immediately after removal of chronic partial coronary stenosis. The increase in use of glucose substrate is of interest and may indicate loss of allosteric regulation by fatty acids or its intermediates upon rate-limiting enzymes in the glycolytic cascade.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume267
Issue number4 36-4
StatePublished - Jan 1 1994

Fingerprint

Coronary Stenosis
Swine
Fatty Acids
Glucose
Reperfusion
Allosteric Regulation
Palmitates
Economic Inflation
Ventricular Pressure
Workload
Myocardium
Pathologic Constriction
Necrosis
Arteries
Perfusion
Heart Rate
Staining and Labeling
Enzymes

Keywords

  • chronic coronary stenosis
  • coronary perfusion
  • coronary reperfusion
  • fatty acids
  • glucose
  • glycolysis

ASJC Scopus subject areas

  • Physiology

Cite this

Myocardial function and metabolism in pig hearts after relief from chronic partial coronary stenosis. / Liedtke, A. J.; Renstrom, B.; Nellis, S. H.; Subramanian, R.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 267, No. 4 36-4, 01.01.1994.

Research output: Contribution to journalArticle

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abstract = "Metabolic behavior was compared during acute extracorporeal reperfusion after removal of a chronic 4-day partial coronary stenosis in eight pig hearts (RCS group) and during comparable extracorporeal perfusion in seven chronically prepared hearts (Sham group). Coronary stenosis in RCS hearts was induced in the left anterior descending (LAD) artery by partial inflation of a hydraulic occluder to restrict LAD peak phasic velocity by ~50{\%}. Regional mechanical shortening was decreased in RCS compared with Sham hearts after 4 days of chronic coronary stenosis [diminished systolic shortening (P < 0.066) with systolic expansion (P < 0.015)] but was comparable to Sham hearts after relief from stenosis. At analogous workloads (left ventricular pressure and heart rate) during reperfusion, metabolic behavior was distinctive between groups. Specifically, compared with Sham hearts, myocardial O2 consumption was selectively increased in RCS hearts (+49Δ{\%}, P < 0.026) as was fatty acid oxidation estimated from 14CO2 production from [U-14C]palmitate (+60Δ{\%}, P < 0.061) and exogenous glucose utilization measured from the release of 3H2O from [5-3H]glucose (+517Δ{\%}, P < 0.025). At the conclusion of the studies, triphenyltetrazolium chloride staining showed no gross evidence of macroinfarction in RCS or Sham hearts, and there was an essentially unremarkable histological survey of anterior myocardium for microscopic necrosis in either group. The level of O2 consumption and preservation of preferred fatty acid utilization indicate that metabolism remains or regains its aerobic pattern of activity in early recovery immediately after removal of chronic partial coronary stenosis. The increase in use of glucose substrate is of interest and may indicate loss of allosteric regulation by fatty acids or its intermediates upon rate-limiting enzymes in the glycolytic cascade.",
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