Myocardial reparative functions of exosomes from mesenchymal stem cells are enhanced by hypoxia treatment of the cells via transferring microRNA-210 in an nSMase2-dependent way

Jinyun Zhu, Kai Lu, Ning Zhang, Yun Zhao, Qunchao Ma, Jian Shen, Yinuo Lin, Pingping Xiang, Yao Liang Tang, Xinyang Hu, Jinghai Chen, Wei Zhu, Keith A. Webster, Jian’an Wang, Hong Yu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hypoxia treatment enhances paracrine effect of mesenchymal stem cells (MSCs). The aim of this study was to investigate whether exosomes from hypoxia-treated MSCs (Exo H ) are superior to those from normoxia-treated MSCs (Exo N ) for myocardial repair. Mouse bone marrow-derived MSCs were cultured under hypoxia or normoxia for 24 h, and exosomes from conditioned media were intramyocardially injected into infarcted heart of C57BL/6 mouse. Exo H resulted in significantly higher survival, smaller scar size and better cardiac functions recovery. Exo H conferred increased vascular density, lower cardiomyocytes (CMs) apoptosis, reduced fibrosis and increased recruitment of cardiac progenitor cells in the infarcted heart relative to Exo N . MicroRNA analysis revealed significantly higher levels of microRNA-210 (miR-210) in Exo H compared with Exo N . Transfection of a miR-210 mimic into endothelial cells (ECs) and CMs conferred similar biological effects as Exo H . Hypoxia treatment of MSCs increased the expression of neutral sphingomyelinase 2 (nSMase2) which is crucial for exosome secretion. Blocking the activity of nSMase2 resulted in reduced miR-210 secretion and abrogated the beneficial effects of Exo H . In conclusion, hypoxic culture augments miR-210 and nSMase2 activities in MSCs and their secreted exosomes, and this is responsible at least in part for the enhanced cardioprotective actions of exosomes derived from hypoxia-treated cells.

Original languageEnglish (US)
Pages (from-to)1659-1670
Number of pages12
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume46
Issue number8
DOIs
StatePublished - Nov 17 2018

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Exosomes
Sphingomyelin Phosphodiesterase
Cell Hypoxia
Stem cells
MicroRNAs
Mesenchymal Stromal Cells
Cells
Cardiac Myocytes
Recovery of Function
Endothelial cells
Cell death
Conditioned Culture Medium
Inbred C57BL Mouse
Cell culture
Cicatrix
Transfection
Blood Vessels
Bone
Fibrosis
Repair

Keywords

  • Exosomes
  • MSCs
  • hypoxia
  • microRNA210
  • myocardial infarction
  • nSMase2

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Myocardial reparative functions of exosomes from mesenchymal stem cells are enhanced by hypoxia treatment of the cells via transferring microRNA-210 in an nSMase2-dependent way. / Zhu, Jinyun; Lu, Kai; Zhang, Ning; Zhao, Yun; Ma, Qunchao; Shen, Jian; Lin, Yinuo; Xiang, Pingping; Tang, Yao Liang; Hu, Xinyang; Chen, Jinghai; Zhu, Wei; Webster, Keith A.; Wang, Jian’an; Yu, Hong.

In: Artificial Cells, Nanomedicine and Biotechnology, Vol. 46, No. 8, 17.11.2018, p. 1659-1670.

Research output: Contribution to journalArticle

Zhu, Jinyun ; Lu, Kai ; Zhang, Ning ; Zhao, Yun ; Ma, Qunchao ; Shen, Jian ; Lin, Yinuo ; Xiang, Pingping ; Tang, Yao Liang ; Hu, Xinyang ; Chen, Jinghai ; Zhu, Wei ; Webster, Keith A. ; Wang, Jian’an ; Yu, Hong. / Myocardial reparative functions of exosomes from mesenchymal stem cells are enhanced by hypoxia treatment of the cells via transferring microRNA-210 in an nSMase2-dependent way. In: Artificial Cells, Nanomedicine and Biotechnology. 2018 ; Vol. 46, No. 8. pp. 1659-1670.
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AU - Xiang, Pingping

AU - Tang, Yao Liang

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AU - Zhu, Wei

AU - Webster, Keith A.

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AB - Hypoxia treatment enhances paracrine effect of mesenchymal stem cells (MSCs). The aim of this study was to investigate whether exosomes from hypoxia-treated MSCs (Exo H ) are superior to those from normoxia-treated MSCs (Exo N ) for myocardial repair. Mouse bone marrow-derived MSCs were cultured under hypoxia or normoxia for 24 h, and exosomes from conditioned media were intramyocardially injected into infarcted heart of C57BL/6 mouse. Exo H resulted in significantly higher survival, smaller scar size and better cardiac functions recovery. Exo H conferred increased vascular density, lower cardiomyocytes (CMs) apoptosis, reduced fibrosis and increased recruitment of cardiac progenitor cells in the infarcted heart relative to Exo N . MicroRNA analysis revealed significantly higher levels of microRNA-210 (miR-210) in Exo H compared with Exo N . Transfection of a miR-210 mimic into endothelial cells (ECs) and CMs conferred similar biological effects as Exo H . Hypoxia treatment of MSCs increased the expression of neutral sphingomyelinase 2 (nSMase2) which is crucial for exosome secretion. Blocking the activity of nSMase2 resulted in reduced miR-210 secretion and abrogated the beneficial effects of Exo H . In conclusion, hypoxic culture augments miR-210 and nSMase2 activities in MSCs and their secreted exosomes, and this is responsible at least in part for the enhanced cardioprotective actions of exosomes derived from hypoxia-treated cells.

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