Myoglobin overexpression inhibits reperfusion in the ischemic mouse hindlimb through impaired angiogenesis but not arteriogenesis

Joshua K. Meisner, Ji Song, Brian H. Annex, Richard J. Price

Research output: Contribution to journalArticle

Abstract

Adaptive vascular remodeling in response to arterial occlusion takes the form of capillary growth (angiogenesis) and outward remodeling of pre-existing collateral arteries (arteriogenesis). However, the relative contributions of angiogenesis and arteriogenesis toward the overall reperfusion response are both highly debated and poorly understood. Here, we tested the hypothesis that myoglobin overexpressing transgenic mice (MbTg+) exhibit impaired angiogenesis in the setting of normal arteriogenesis in response to femoral artery ligation, and thereby serve as a model for disconnecting these two vascular growth processes. After femoral artery ligation, MbTg+ mice were characterized by delayed distal limb reperfusion (by laser Doppler perfusion imaging), decreased foot use, and impaired distal limb muscle angiogenesis in both glycolytic and oxidative muscle fiber regions at day 7. Substantial arteriogenesis occurred in the primary collaterals supplying the ischemic limb in both wild-type and MbTg+ mice; however, there were no significant differences between groups, indicating that myoglobin overexpression does not affect arteriogenesis. Together, these results uniquely demonstrate that functional collateral arteriogenesis alone is not necessarily sufficient for adequate reperfusion after arterial occlusion. Angiogenesis is a key component of an effective reperfusion response, and clinical strategies that target both angiogenesis and arteriogenesis could yield the most efficacious treatments for peripheral arterial disease.

Original languageEnglish (US)
Pages (from-to)1710-1718
Number of pages9
JournalAmerican Journal of Pathology
Volume183
Issue number6
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

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Myoglobin
Hindlimb
Reperfusion
Extremities
Femoral Artery
Ligation
Muscles
Perfusion Imaging
Peripheral Arterial Disease
Growth
Transgenic Mice
Blood Vessels
Foot
Lasers

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Myoglobin overexpression inhibits reperfusion in the ischemic mouse hindlimb through impaired angiogenesis but not arteriogenesis. / Meisner, Joshua K.; Song, Ji; Annex, Brian H.; Price, Richard J.

In: American Journal of Pathology, Vol. 183, No. 6, 01.12.2013, p. 1710-1718.

Research output: Contribution to journalArticle

Meisner, Joshua K. ; Song, Ji ; Annex, Brian H. ; Price, Richard J. / Myoglobin overexpression inhibits reperfusion in the ischemic mouse hindlimb through impaired angiogenesis but not arteriogenesis. In: American Journal of Pathology. 2013 ; Vol. 183, No. 6. pp. 1710-1718.
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