Myosin 16 levels fluctuate during the cell cycle and are downregulated in response to DNA replication stress

Richard S Cameron, Changdan Liu, Jeanene P.S. Pihkala

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Myosins comprise a highly conserved superfamily of eukaryotic actin-dependent motor proteins implicated in a large repertoire of functions in both the cytoplasm and the nucleus. Class XVI myosin, MYO16, reveals expression in most somatic as well as meiotic cells with prominent localization in the nucleus, excepting the nucleolus; however, the role(s) of Myo16 in the nucleus remain unknown. In this report, we investigated Myo16 abundance during transit through the cell cycle. Immunolocalization, immunoblot, flow cytometric and quantitative RT-PCR studies performed in Rat2 cells indicate that Myo16 mRNA and protein abundance are cell cycle regulated: in the unperturbed cell cycle, each rises to peak levels in late G1 and thereon through S-phase and each decays as cells enter M-phase. Notably, RNA interference-induced Myo16 depletion results in altered cell cycle distribution as well as in large-scale cell death. In response to DNA replication stress (impaired replication fork progression as a consequence of DNA damage, lack of sufficient deoxynucleotides, or inhibition of DNA polymerases), Myo16 protein shows substantial loss. Attenuation of replication stress (aphidicolin or hydroxyurea) is followed by a recovery of Myo16 expression and resumption of S-phase progression. Collectively, these observations suggest that Myo16 may play a regulatory role in cell cycle progression.

Original languageEnglish (US)
Pages (from-to)328-348
Number of pages21
JournalCytoskeleton
Volume70
Issue number6
DOIs
StatePublished - Jun 1 2013

Fingerprint

Myosins
DNA Replication
Cell Cycle
Down-Regulation
S Phase
Aphidicolin
Cell Cycle Proteins
Hydroxyurea
DNA-Directed DNA Polymerase
RNA Interference
Cell Division
DNA Damage
Actins
Cytoplasm
Proteins
Cell Death
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cell cycle
  • DNA damage response
  • Myosin XVI
  • Nucleus

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

Cite this

Myosin 16 levels fluctuate during the cell cycle and are downregulated in response to DNA replication stress. / Cameron, Richard S; Liu, Changdan; Pihkala, Jeanene P.S.

In: Cytoskeleton, Vol. 70, No. 6, 01.06.2013, p. 328-348.

Research output: Contribution to journalArticle

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