Myosin X regulates neuronal radial migration through interacting with N-cadherin

Mingming Lai, Ye Guo, Jun Ma, Huali Yu, Dongdong Zhao, Wenqiang Fan, Xingda Ju, Muhammad A. Sheikh, Yousra S. Malik, Wencheng Xiong, Weixiang Guo, Xiaojuan Zhu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Proper brain function depends on correct neuronal migration during development, which is known to be regulated by cytoskeletal dynamics and cell-cell adhesion. Myosin X (Myo10), an uncharacteristic member of the myosin family, is an important regulator of cytoskeleton that modulates cell motilities in many different cellular contexts. We previously reported that Myo10 was required for neuronal migration in the developing cerebral cortex, but the underlying mechanism was still largely unknown. Here, we found that knockdown of Myo10 expression disturbed the adherence of migrating neurons to radial glial fibers through abolishing surface Neuronal cadherin (N-cadherin) expression, thereby impaired neuronal migration in the developmental cortex. Next, we found Myo10 interacted with N-cadherin cellular domain through its FERM domain. Furthermore, we found knockdown of Myo10 disrupted N-cadherin subcellular distribution and led to localization of N-cadherin into Golgi apparatus and endosomal sorting vesicle. Taking together, these results reveal a novel mechanism of Myo10 interacting with N-cadherin and regulating its cell-surface expression, which is required for neuronal adhesion and migration.

Original languageEnglish (US)
Article number326
JournalFrontiers in Cellular Neuroscience
Volume9
Issue numberAUGUST
DOIs
StatePublished - Aug 18 2015

Fingerprint

Cadherins
Myosins
Golgi Apparatus
Cytoskeleton
Cell Adhesion
Neuroglia
Cerebral Cortex
Cell Movement
Neurons
Brain

Keywords

  • Cell adhesion
  • Interaction
  • Membrane trafficking
  • Myo10
  • N-cadherin
  • Neuronal migration

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Myosin X regulates neuronal radial migration through interacting with N-cadherin. / Lai, Mingming; Guo, Ye; Ma, Jun; Yu, Huali; Zhao, Dongdong; Fan, Wenqiang; Ju, Xingda; Sheikh, Muhammad A.; Malik, Yousra S.; Xiong, Wencheng; Guo, Weixiang; Zhu, Xiaojuan.

In: Frontiers in Cellular Neuroscience, Vol. 9, No. AUGUST, 326, 18.08.2015.

Research output: Contribution to journalArticle

Lai, M, Guo, Y, Ma, J, Yu, H, Zhao, D, Fan, W, Ju, X, Sheikh, MA, Malik, YS, Xiong, W, Guo, W & Zhu, X 2015, 'Myosin X regulates neuronal radial migration through interacting with N-cadherin', Frontiers in Cellular Neuroscience, vol. 9, no. AUGUST, 326. https://doi.org/10.3389/fncel.2015.00326
Lai, Mingming ; Guo, Ye ; Ma, Jun ; Yu, Huali ; Zhao, Dongdong ; Fan, Wenqiang ; Ju, Xingda ; Sheikh, Muhammad A. ; Malik, Yousra S. ; Xiong, Wencheng ; Guo, Weixiang ; Zhu, Xiaojuan. / Myosin X regulates neuronal radial migration through interacting with N-cadherin. In: Frontiers in Cellular Neuroscience. 2015 ; Vol. 9, No. AUGUST.
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