Abstract
Directed neuronal, astroglial, and oligodendroglial cell migrations comprise a prominent feature of mammalian brain development. Because molecular motor proteins have been implicated in a wide spectrum of processes associated with cell motility, we initiated studies to define the pool of myosins in migrating cerebellar granule neurons and type-1 neocortical astrocytes. Our analyses identified two isoforms of a novel unconventional myosin, which we have cloned, sequenced, and designated myr 8a and 8b (eighth unconventional myosin from rat). Phylogenetic analysis indicates that myr 8 myosins comprise a new class of myosins, which we have designated class XVI. The head domain contains a large N-terminal extension composed of multiple ankyrin repeats, which are implicated in mediating an association with the protein phosphatase 1 (PP1) catalytic subunits 1α and 1γ. The motor domain is followed by a single putative light-chain binding domain. The tail domain of myr 8a is comparatively short with a net positive charge, whereas the tail domain of myr 8b is extended, bears an overall neutral charge, and reveals several stretches of polyproline residues. Neither the myr 8a nor the myr 8b sequence reveals α-helical coiled-coil motifs, suggesting that these myosins exist as monomers. Both immunoblot and Northern blot analyses indicate that myr 8b is the predominant isoform expressed in brain, principally at developmental time periods. The structural features and restricted expression patterns suggest that members of this novel class of unconventional myosins comprise a mechanism to target selectively the protein phosphatase 1 catalytic subunits 1α and/or 1γ in developing brain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7954-7968 |
| Number of pages | 15 |
| Journal | Journal of Neuroscience |
| Volume | 21 |
| Issue number | 20 |
| State | Published - Oct 15 2001 |
Fingerprint
Keywords
- Astrocytes
- Brain development
- Cytoskeleton
- Nervous system
- Neuronal migration
- Unconventional myosins
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Myr 8, a novel unconventional myosin expressed during brain development associates with the protein phosphatase catalytic subunits 1α and 1γ1. / Patel, Krishna G.; Liu, Changdan; Cameron, Patricia L.; Cameron, Richard S.
In: Journal of Neuroscience, Vol. 21, No. 20, 15.10.2001, p. 7954-7968.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Myr 8, a novel unconventional myosin expressed during brain development associates with the protein phosphatase catalytic subunits 1α and 1γ1
AU - Patel, Krishna G.
AU - Liu, Changdan
AU - Cameron, Patricia L.
AU - Cameron, Richard S.
PY - 2001/10/15
Y1 - 2001/10/15
N2 - Directed neuronal, astroglial, and oligodendroglial cell migrations comprise a prominent feature of mammalian brain development. Because molecular motor proteins have been implicated in a wide spectrum of processes associated with cell motility, we initiated studies to define the pool of myosins in migrating cerebellar granule neurons and type-1 neocortical astrocytes. Our analyses identified two isoforms of a novel unconventional myosin, which we have cloned, sequenced, and designated myr 8a and 8b (eighth unconventional myosin from rat). Phylogenetic analysis indicates that myr 8 myosins comprise a new class of myosins, which we have designated class XVI. The head domain contains a large N-terminal extension composed of multiple ankyrin repeats, which are implicated in mediating an association with the protein phosphatase 1 (PP1) catalytic subunits 1α and 1γ. The motor domain is followed by a single putative light-chain binding domain. The tail domain of myr 8a is comparatively short with a net positive charge, whereas the tail domain of myr 8b is extended, bears an overall neutral charge, and reveals several stretches of polyproline residues. Neither the myr 8a nor the myr 8b sequence reveals α-helical coiled-coil motifs, suggesting that these myosins exist as monomers. Both immunoblot and Northern blot analyses indicate that myr 8b is the predominant isoform expressed in brain, principally at developmental time periods. The structural features and restricted expression patterns suggest that members of this novel class of unconventional myosins comprise a mechanism to target selectively the protein phosphatase 1 catalytic subunits 1α and/or 1γ in developing brain.
AB - Directed neuronal, astroglial, and oligodendroglial cell migrations comprise a prominent feature of mammalian brain development. Because molecular motor proteins have been implicated in a wide spectrum of processes associated with cell motility, we initiated studies to define the pool of myosins in migrating cerebellar granule neurons and type-1 neocortical astrocytes. Our analyses identified two isoforms of a novel unconventional myosin, which we have cloned, sequenced, and designated myr 8a and 8b (eighth unconventional myosin from rat). Phylogenetic analysis indicates that myr 8 myosins comprise a new class of myosins, which we have designated class XVI. The head domain contains a large N-terminal extension composed of multiple ankyrin repeats, which are implicated in mediating an association with the protein phosphatase 1 (PP1) catalytic subunits 1α and 1γ. The motor domain is followed by a single putative light-chain binding domain. The tail domain of myr 8a is comparatively short with a net positive charge, whereas the tail domain of myr 8b is extended, bears an overall neutral charge, and reveals several stretches of polyproline residues. Neither the myr 8a nor the myr 8b sequence reveals α-helical coiled-coil motifs, suggesting that these myosins exist as monomers. Both immunoblot and Northern blot analyses indicate that myr 8b is the predominant isoform expressed in brain, principally at developmental time periods. The structural features and restricted expression patterns suggest that members of this novel class of unconventional myosins comprise a mechanism to target selectively the protein phosphatase 1 catalytic subunits 1α and/or 1γ in developing brain.
KW - Astrocytes
KW - Brain development
KW - Cytoskeleton
KW - Nervous system
KW - Neuronal migration
KW - Unconventional myosins
UR - http://www.scopus.com/inward/record.url?scp=0035887589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035887589&partnerID=8YFLogxK
M3 - Article
C2 - 11588169
AN - SCOPUS:0035887589
VL - 21
SP - 7954
EP - 7968
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 20
ER -