NADH/NADPH oxidase and enhanced superoxide production in the mineralocorticoid hypertensive rat

Richard A. Beswick, Anne M. Dorrance, Romulo Leite, R. Clinton Webb

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

We previously reported increased aortic reactive oxygen species (ROS) production in mineralocorticoid (deoxycorticosterone acetate [DOCA]-salt) hypertensive rats. In the present study, we tested the hypothesis that NADH/NADPH oxidase is responsible for increased ROS production, namely superoxide (O2-), in aorta from the DOCA-salt rat. Treatment of aortic rings from DOCA-salt rats with the NO synthase inhibitor N-nitro-L-arginine and the xanthine oxidase inhibitor allopurinol did not significantly change O2-- production. Furthermore, de-endothelialization of aorta from DOCA-salt rats did not affect O2- production compared with that of sham-operated rats. Thus, xanthine oxidase and uncoupled endothelial NO synthase were not responsible for increased O2- production in the DOCA-salt rats. In contrast, treatment with the NADPH oxidase inhibitor apocynin significantly decreased O2- production in aortic rings from DOCA-salt rats compared with sham-operated rats. Moreover, long-term administration of apocynin (in drinking water, 1.5 mmol/L, 28 days) to DOCA-salt rats significantly decreased systolic blood pressure compared with that of rats treated with DOCA-salt alone. Furthermore, O2- production in aortic rings from DOCA-salt rats treated with apocynin for 28 days was reduced compared with that of untreated DOCA-salt rats. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that DOCA-salt rats have significantly greater mRNA levels of the NADPH oxidase subunit p22phox than do sham-operated rats. These findings suggest that NADPH oxidase is increased and is responsible for increased O2- production and possibly contributes to increased blood pressure in the DOCA-salt hypertensive rat.

Original languageEnglish (US)
Pages (from-to)1107-1111
Number of pages5
JournalHypertension
Volume38
Issue number5
DOIs
StatePublished - Jan 1 2001

Fingerprint

Mineralocorticoids
NADPH Oxidase
Desoxycorticosterone
Superoxides
Acetates
Salts
Xanthine Oxidase
NADH oxidase
Blood Pressure
Nitric Oxide Synthase
Aorta
Reactive Oxygen Species
Allopurinol
Reverse Transcriptase Polymerase Chain Reaction
Drinking Water

Keywords

  • Deoxycorticosterone acetate
  • Hypertension, mineralocorticoid
  • Mineralocorticoids
  • NADH/NADPH

ASJC Scopus subject areas

  • Internal Medicine

Cite this

NADH/NADPH oxidase and enhanced superoxide production in the mineralocorticoid hypertensive rat. / Beswick, Richard A.; Dorrance, Anne M.; Leite, Romulo; Webb, R. Clinton.

In: Hypertension, Vol. 38, No. 5, 01.01.2001, p. 1107-1111.

Research output: Contribution to journalArticle

Beswick, Richard A. ; Dorrance, Anne M. ; Leite, Romulo ; Webb, R. Clinton. / NADH/NADPH oxidase and enhanced superoxide production in the mineralocorticoid hypertensive rat. In: Hypertension. 2001 ; Vol. 38, No. 5. pp. 1107-1111.
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