Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats

Marília Afonso Rabelo Buzalaf, Elide Escolástico Caroselli, Rodrigo Cardoso De Oliveira, José Mauro Granjeiro, Gary M. Whitford

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

When acute exposure to fluoride is thought to be the cause of death, confirmation often depends on the analysis of some body fluid or tissue. The aim of this study was to evaluate the use of nails and the periosteal surface of bone as indicators of acute exposure to fluoride. Six groups of rats were given a single oral dose of fluoride (50 mg/kg body weight), while the control group was given deionized water. The rats were killed at 2, 4, 8, 16, 24, and 48 h after fluoride administration. Plasma and nails (the proximal halves) were collected and analyzed for fluoride with an ion-specific electrode after hexamethyldisiloxane-facilitated diffusion. A circular area of the femur (4.52 mm2) was etched with 0.5M HCl for 15 s, and, after the addition of a buffer, the solution was analyzed with an ion-specific electrode. Peak plasma concentration occurred at 2 h, followed by progressively declining concentrations. Peak nail fluoride concentrations occurred at 8 h. The mean nail concentrations at 8, 16, and 24 h were significantly higher than that of the control group. Bone surface concentrations were significantly higher than that of the control group at 4 h and thereafter. Thus, the proximal portion of nails and bone surface are suitable biomarkers for acute fluoride exposure in rats.

Original languageEnglish (US)
Pages (from-to)249-252
Number of pages4
JournalJournal of Analytical Toxicology
Volume28
Issue number4
StatePublished - May 1 2004

Fingerprint

Bone Nails
Nails
Biomarkers
Fluorides
fluoride
biomarker
Rats
bone
Bone
Control Groups
Plasmas
Electrodes
electrode
Deionized water
Body fluids
Ions
Facilitated Diffusion
plasma
Bone and Bones
body fluid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

Rabelo Buzalaf, M. A., Caroselli, E. E., Cardoso De Oliveira, R., Granjeiro, J. M., & Whitford, G. M. (2004). Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats. Journal of Analytical Toxicology, 28(4), 249-252.

Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats. / Rabelo Buzalaf, Marília Afonso; Caroselli, Elide Escolástico; Cardoso De Oliveira, Rodrigo; Granjeiro, José Mauro; Whitford, Gary M.

In: Journal of Analytical Toxicology, Vol. 28, No. 4, 01.05.2004, p. 249-252.

Research output: Contribution to journalArticle

Rabelo Buzalaf, MA, Caroselli, EE, Cardoso De Oliveira, R, Granjeiro, JM & Whitford, GM 2004, 'Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats', Journal of Analytical Toxicology, vol. 28, no. 4, pp. 249-252.
Rabelo Buzalaf MA, Caroselli EE, Cardoso De Oliveira R, Granjeiro JM, Whitford GM. Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats. Journal of Analytical Toxicology. 2004 May 1;28(4):249-252.
Rabelo Buzalaf, Marília Afonso ; Caroselli, Elide Escolástico ; Cardoso De Oliveira, Rodrigo ; Granjeiro, José Mauro ; Whitford, Gary M. / Nail and Bone Surface as Biomarkers for Acute Fluoride Exposure in Rats. In: Journal of Analytical Toxicology. 2004 ; Vol. 28, No. 4. pp. 249-252.
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