Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells

Zhichuan Li, Zhongbing Zhang, Joe X. Xie, Xin Li, Jiang Tian, Ting Cai, Hongjuan Cui, Hanfei Ding, Joseph I. Shapiro, Zijian Xie

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Cells contain a large pool of nonpumping Na/K-ATPase that participates in signal transduction. Here, we show that the expression of α1 Na/K-ATPase is significantly reduced in human prostate carcinoma as well as in several human cancer cell lines. This down-regulation impairs the ability of Na/KATPase to regulate Src-related signaling processes. A supplement of pNaKtide, a peptide derived from α1 Na/K-ATPase, reduces the activities of Src and Src effectors. Consequently, these treatments stimulate apoptosis and inhibit growth in cultures of human cancer cells. Moreover, administration of pNaKtide inhibits angiogenesis and growth of tumor xenograft. Thus, the new findings demonstrate the in vivo effectiveness of pNaKtide and suggest that the defect in Na/K-ATPase-mediated signal transduction may be targeted for developing new anticancer therapeutics.

Original languageEnglish (US)
Pages (from-to)32394-32403
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number37
DOIs
StatePublished - Sep 16 2011

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Adenosine Triphosphatases
Cells
Signal transduction
Peptides
Growth
Signal Transduction
Neoplasms
Cell culture
Heterografts
Tumors
Prostate
Down-Regulation
Apoptosis
Carcinoma
Cell Line
Defects
sodium-translocating ATPase
Therapeutics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Li, Z., Zhang, Z., Xie, J. X., Li, X., Tian, J., Cai, T., ... Xie, Z. (2011). Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells. Journal of Biological Chemistry, 286(37), 32394-32403. https://doi.org/10.1074/jbc.M110.207597

Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells. / Li, Zhichuan; Zhang, Zhongbing; Xie, Joe X.; Li, Xin; Tian, Jiang; Cai, Ting; Cui, Hongjuan; Ding, Hanfei; Shapiro, Joseph I.; Xie, Zijian.

In: Journal of Biological Chemistry, Vol. 286, No. 37, 16.09.2011, p. 32394-32403.

Research output: Contribution to journalArticle

Li, Z, Zhang, Z, Xie, JX, Li, X, Tian, J, Cai, T, Cui, H, Ding, H, Shapiro, JI & Xie, Z 2011, 'Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells', Journal of Biological Chemistry, vol. 286, no. 37, pp. 32394-32403. https://doi.org/10.1074/jbc.M110.207597
Li, Zhichuan ; Zhang, Zhongbing ; Xie, Joe X. ; Li, Xin ; Tian, Jiang ; Cai, Ting ; Cui, Hongjuan ; Ding, Hanfei ; Shapiro, Joseph I. ; Xie, Zijian. / Na/K-ATPase mimetic pNaKtide peptide inhibits the growth of human cancer cells. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 37. pp. 32394-32403.
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