Nanoleakage at the dentin adhesive interface: A new application for laser ablation-sector field-ICPMS

A. Mohamad Ghazi, Stephen Shuttleworth, Robert Simmons, S. John Paul, David H. Pashley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Laser ablation-sector field-inductively coupled plasma mass spectrometry has been used for in situ determination of silver concentration in treated human teeth. Silver nitrate has long been used to trace the presence of micro sized gaps between the walls of dental restorations and teeth, and more recently, to trace the presence of nanometer sized voids within resin-bonded restorations. This study examines the diffusion of silver nitrate into submicron voids beneath resin-bonded composite restorations. The silver treated-teeth were analyzed for 43Ca, 107Ag, and 109Ag isotopes. Elemental data were obtained by ablation with Nd:YAG laser operating in the UV region 266 nm and 213 nm by New Wave-Merchantek and isotopic analysis with a sector field ICP MS by Finnigan MAT. A 50 ppm glass standard (NIST-612) was also analyzed for the same isotopes. Quantification was performed by assuming a uniform nominal calcium concentration value of 27 g per 100 g of dry dentin across the root and using the 43Ca signal for internal calibration. The precision (RSD%) of measurements for 43Ca for the NIST-612 glass standard was found to be within 1.8% to 2.5% and for the tooth samples within 2.8% to 7.1%. The precision of analysis for 107Ag for the NIST-612 glass standard was between 2.6% and 4.4%. The analysis revealed a significant penetration of resin-bonded dentin by silver, ranging from ∼35000 ppm at the very bottom of the resin-infiltrated demineralized dentin to only 10 s of ppm in the areas within the underlying mineralized dentin. The analysis revealed that a significant amount of silver penetrated through resin-dentin bonds, and that the amount of silver increased with increased acid-etching time. The results from this study suggest that current adhesive resin system do not create perfect bonds and that laser ablation sector field ICPMS can be used to quantify the amount of nanoleakage of bonded restorations.

Original languageEnglish (US)
Pages (from-to)682-687
Number of pages6
JournalJournal of analytical atomic spectrometry
Volume17
Issue number7
DOIs
StatePublished - Jan 1 2002

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Laser ablation
Adhesives
Silver
Resins
Restoration
Silver Nitrate
Isotopes
Glass
Inductively coupled plasma mass spectrometry
Ablation
Etching
Calibration
Calcium
Acids
Lasers
Composite materials

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Nanoleakage at the dentin adhesive interface : A new application for laser ablation-sector field-ICPMS. / Mohamad Ghazi, A.; Shuttleworth, Stephen; Simmons, Robert; Paul, S. John; Pashley, David H.

In: Journal of analytical atomic spectrometry, Vol. 17, No. 7, 01.01.2002, p. 682-687.

Research output: Contribution to journalArticle

Mohamad Ghazi, A. ; Shuttleworth, Stephen ; Simmons, Robert ; Paul, S. John ; Pashley, David H. / Nanoleakage at the dentin adhesive interface : A new application for laser ablation-sector field-ICPMS. In: Journal of analytical atomic spectrometry. 2002 ; Vol. 17, No. 7. pp. 682-687.
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abstract = "Laser ablation-sector field-inductively coupled plasma mass spectrometry has been used for in situ determination of silver concentration in treated human teeth. Silver nitrate has long been used to trace the presence of micro sized gaps between the walls of dental restorations and teeth, and more recently, to trace the presence of nanometer sized voids within resin-bonded restorations. This study examines the diffusion of silver nitrate into submicron voids beneath resin-bonded composite restorations. The silver treated-teeth were analyzed for 43Ca, 107Ag, and 109Ag isotopes. Elemental data were obtained by ablation with Nd:YAG laser operating in the UV region 266 nm and 213 nm by New Wave-Merchantek and isotopic analysis with a sector field ICP MS by Finnigan MAT. A 50 ppm glass standard (NIST-612) was also analyzed for the same isotopes. Quantification was performed by assuming a uniform nominal calcium concentration value of 27 g per 100 g of dry dentin across the root and using the 43Ca signal for internal calibration. The precision (RSD{\%}) of measurements for 43Ca for the NIST-612 glass standard was found to be within 1.8{\%} to 2.5{\%} and for the tooth samples within 2.8{\%} to 7.1{\%}. The precision of analysis for 107Ag for the NIST-612 glass standard was between 2.6{\%} and 4.4{\%}. The analysis revealed a significant penetration of resin-bonded dentin by silver, ranging from ∼35000 ppm at the very bottom of the resin-infiltrated demineralized dentin to only 10 s of ppm in the areas within the underlying mineralized dentin. The analysis revealed that a significant amount of silver penetrated through resin-dentin bonds, and that the amount of silver increased with increased acid-etching time. The results from this study suggest that current adhesive resin system do not create perfect bonds and that laser ablation sector field ICPMS can be used to quantify the amount of nanoleakage of bonded restorations.",
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AB - Laser ablation-sector field-inductively coupled plasma mass spectrometry has been used for in situ determination of silver concentration in treated human teeth. Silver nitrate has long been used to trace the presence of micro sized gaps between the walls of dental restorations and teeth, and more recently, to trace the presence of nanometer sized voids within resin-bonded restorations. This study examines the diffusion of silver nitrate into submicron voids beneath resin-bonded composite restorations. The silver treated-teeth were analyzed for 43Ca, 107Ag, and 109Ag isotopes. Elemental data were obtained by ablation with Nd:YAG laser operating in the UV region 266 nm and 213 nm by New Wave-Merchantek and isotopic analysis with a sector field ICP MS by Finnigan MAT. A 50 ppm glass standard (NIST-612) was also analyzed for the same isotopes. Quantification was performed by assuming a uniform nominal calcium concentration value of 27 g per 100 g of dry dentin across the root and using the 43Ca signal for internal calibration. The precision (RSD%) of measurements for 43Ca for the NIST-612 glass standard was found to be within 1.8% to 2.5% and for the tooth samples within 2.8% to 7.1%. The precision of analysis for 107Ag for the NIST-612 glass standard was between 2.6% and 4.4%. The analysis revealed a significant penetration of resin-bonded dentin by silver, ranging from ∼35000 ppm at the very bottom of the resin-infiltrated demineralized dentin to only 10 s of ppm in the areas within the underlying mineralized dentin. The analysis revealed that a significant amount of silver penetrated through resin-dentin bonds, and that the amount of silver increased with increased acid-etching time. The results from this study suggest that current adhesive resin system do not create perfect bonds and that laser ablation sector field ICPMS can be used to quantify the amount of nanoleakage of bonded restorations.

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