Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms

Bridget M. Messaros, Mary G. Rossano, Guanghui Liu, Michael Peter Diamond, Karen Friderici, Katherine Nummy-Jernigan, Douglas Daly, Elizabeth Puscheck, Nigel Paneth, Julia J. Wirth

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated. Methods: Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20 million/mL, motility <50%, morphology <4%) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides. Results: High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95% confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms. Conclusion: High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.

Original languageEnglish (US)
Pages (from-to)457-464
Number of pages8
JournalEnvironmental Research
Volume109
Issue number4
DOIs
StatePublished - May 1 2009
Externally publishedYes

Fingerprint

Dichlorodiphenyl Dichloroethylene
DDT
Genetic Polymorphisms
DDE
Polymorphism
sperm
Spermatozoa
serum
polymorphism
Cytochrome P-450 CYP1A1
Serum
Odds Ratio
allele
Alleles
motility
demographic method
Logistic Models
infertility
effect
parameter

Keywords

  • CYP1A1
  • GSTM1
  • GSTT1
  • Genetic polymorphisms
  • Human exposure
  • Sperm
  • p,p′-DDE

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

Cite this

Messaros, B. M., Rossano, M. G., Liu, G., Diamond, M. P., Friderici, K., Nummy-Jernigan, K., ... Wirth, J. J. (2009). Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms. Environmental Research, 109(4), 457-464. https://doi.org/10.1016/j.envres.2009.02.009

Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms. / Messaros, Bridget M.; Rossano, Mary G.; Liu, Guanghui; Diamond, Michael Peter; Friderici, Karen; Nummy-Jernigan, Katherine; Daly, Douglas; Puscheck, Elizabeth; Paneth, Nigel; Wirth, Julia J.

In: Environmental Research, Vol. 109, No. 4, 01.05.2009, p. 457-464.

Research output: Contribution to journalArticle

Messaros, BM, Rossano, MG, Liu, G, Diamond, MP, Friderici, K, Nummy-Jernigan, K, Daly, D, Puscheck, E, Paneth, N & Wirth, JJ 2009, 'Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms', Environmental Research, vol. 109, no. 4, pp. 457-464. https://doi.org/10.1016/j.envres.2009.02.009
Messaros, Bridget M. ; Rossano, Mary G. ; Liu, Guanghui ; Diamond, Michael Peter ; Friderici, Karen ; Nummy-Jernigan, Katherine ; Daly, Douglas ; Puscheck, Elizabeth ; Paneth, Nigel ; Wirth, Julia J. / Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms. In: Environmental Research. 2009 ; Vol. 109, No. 4. pp. 457-464.
@article{b943b38f33c8425f9aa88ad4be90735a,
title = "Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms",
abstract = "Objective: Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated. Methods: Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20 million/mL, motility <50{\%}, morphology <4{\%}) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides. Results: High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95{\%} confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms. Conclusion: High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.",
keywords = "CYP1A1, GSTM1, GSTT1, Genetic polymorphisms, Human exposure, Sperm, p,p′-DDE",
author = "Messaros, {Bridget M.} and Rossano, {Mary G.} and Guanghui Liu and Diamond, {Michael Peter} and Karen Friderici and Katherine Nummy-Jernigan and Douglas Daly and Elizabeth Puscheck and Nigel Paneth and Wirth, {Julia J.}",
year = "2009",
month = "5",
day = "1",
doi = "10.1016/j.envres.2009.02.009",
language = "English (US)",
volume = "109",
pages = "457--464",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms

AU - Messaros, Bridget M.

AU - Rossano, Mary G.

AU - Liu, Guanghui

AU - Diamond, Michael Peter

AU - Friderici, Karen

AU - Nummy-Jernigan, Katherine

AU - Daly, Douglas

AU - Puscheck, Elizabeth

AU - Paneth, Nigel

AU - Wirth, Julia J.

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Objective: Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated. Methods: Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20 million/mL, motility <50%, morphology <4%) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides. Results: High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95% confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms. Conclusion: High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.

AB - Objective: Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated. Methods: Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20 million/mL, motility <50%, morphology <4%) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides. Results: High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95% confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms. Conclusion: High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.

KW - CYP1A1

KW - GSTM1

KW - GSTT1

KW - Genetic polymorphisms

KW - Human exposure

KW - Sperm

KW - p,p′-DDE

UR - http://www.scopus.com/inward/record.url?scp=64749114267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64749114267&partnerID=8YFLogxK

U2 - 10.1016/j.envres.2009.02.009

DO - 10.1016/j.envres.2009.02.009

M3 - Article

VL - 109

SP - 457

EP - 464

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

IS - 4

ER -