Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms

Bridget M. Messaros, Mary G. Rossano, Guanghui Liu, Michael P. Diamond, Karen Friderici, Katherine Nummy-Jernigan, Douglas Daly, Elizabeth Puscheck, Nigel Paneth, Julia J. Wirth

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Objective: Effects of ambient exposure to DDT and its metabolites (DDE-DDT) on human sperm parameters and the role of genetic polymorphisms in modifying the association were investigated. Methods: Demographics, medical history data, blood and semen samples were obtained from the first 336 male partners of couples presenting to 2 infertility clinics. Serum was analyzed for organochlorines (OC) and DNA for polymorphisms in GSTM1, GSTT1, GSTP1 and CYP1A1. Men with each sperm parameter considered low by WHO criteria (concentration <20 million/mL, motility <50%, morphology <4%) were compared to men with all normal sperm parameters in logistic regression models, controlling for sum of other OC pesticides. Results: High DDE-DDT level was associated with significantly increased odds for all 3 low sperm parameters. The risk of low motility with high DDE-DDT exposure was increased in men with the GSTT1 null genotype compared to those with GSTT1 intact (odds ratio (OR)=4.19, 95% confidence interval (CI) 1.05-16.78 and OR=3.57, 1.43-8.93, respectively). Risk for low morphology in men with high DDE-DDT and one or both CYP1A1*2A alleles was lower compared to men with the common CYP1A1 alleles (OR=2.18, 0.78-6.07 vs. OR=3.45, 1.32-9.03, respectively). Similar results were obtained for men with low DDE-DDT exposure. Effects of high DDE-DDT on low sperm concentration (OR=2.53, 1.0-6.31) was unaffected by the presence of the polymorphisms. Conclusion: High DDE-DDT exposure adversely affected all 3 sperm parameters and its effects were exacerbated by the GSTT1 null polymorphism and by the CYP1A1 common alleles.

Original languageEnglish (US)
Pages (from-to)457-464
Number of pages8
JournalEnvironmental Research
Volume109
Issue number4
DOIs
StatePublished - May 2009

Keywords

  • CYP1A1
  • GSTM1
  • GSTT1
  • Genetic polymorphisms
  • Human exposure
  • Sperm
  • p,p′-DDE

ASJC Scopus subject areas

  • Biochemistry
  • Environmental Science(all)

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    Messaros, B. M., Rossano, M. G., Liu, G., Diamond, M. P., Friderici, K., Nummy-Jernigan, K., Daly, D., Puscheck, E., Paneth, N., & Wirth, J. J. (2009). Negative effects of serum p,p′-DDE on sperm parameters and modification by genetic polymorphisms. Environmental Research, 109(4), 457-464. https://doi.org/10.1016/j.envres.2009.02.009