Abstract
Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3′-5′ exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-59 |
| Number of pages | 17 |
| Journal | Protein and Cell |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- Dis3l2
- Schmidtea mediterranea
- adult stem cells
- local proliferation
- planarians
- wound recognition
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Drug Discovery
- Cell Biology
Cite this
Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration. / Han, Xiao Shuai; Wang, Chen; Guo, Fang hao; Huang, Shuang; Qin, Yong Wen; Zhao, Xian Xian; Jing, Qing.
In: Protein and Cell, Vol. 10, No. 1, 01.01.2019, p. 43-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration
AU - Han, Xiao Shuai
AU - Wang, Chen
AU - Guo, Fang hao
AU - Huang, Shuang
AU - Qin, Yong Wen
AU - Zhao, Xian Xian
AU - Jing, Qing
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3′-5′ exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.
AB - Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3′-5′ exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.
KW - Dis3l2
KW - Schmidtea mediterranea
KW - adult stem cells
KW - local proliferation
KW - planarians
KW - wound recognition
UR - http://www.scopus.com/inward/record.url?scp=85046035335&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046035335&partnerID=8YFLogxK
U2 - 10.1007/s13238-018-0512-0
DO - 10.1007/s13238-018-0512-0
M3 - Article
AN - SCOPUS:85046035335
VL - 10
SP - 43
EP - 59
JO - Protein and Cell
JF - Protein and Cell
SN - 1674-800X
IS - 1
ER -