@article{dfedf02abc854cd28bd008da0655c649,
title = "Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration",
abstract = "Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3′-5′ exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.",
keywords = "Dis3l2, Schmidtea mediterranea, adult stem cells, local proliferation, planarians, wound recognition",
author = "Han, {Xiao Shuai} and Chen Wang and Guo, {Fang hao} and Shuang Huang and Qin, {Yong Wen} and Zhao, {Xian Xian} and Qing Jing",
note = "Funding Information: We thank P. Newmark, P. Reddien, and N. Oviedo for kindly providing worms. We thank S. Lapan, D. Wenemoser, and K. Kravarik for FISH advice, J. Wolfswinkel for FACS assistance. We thank X. Qiu and J. Chen for critically reading the manuscript. We thank all members of Jing lab for comments and discussion. We thank the staff in the core facility (Institute of Health Sciences) for technical assistance. This work was supported in part by the National Key Research and Development Program of China (2017YFA0103700), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020903), and the National Natural Science Foundation of China (91739301, 91339205, and 31229002). Funding Information: We thank P. Newmark, P. Reddien, and N. Oviedo for kindly providing worms. We thank S. Lapan, D. Wenemoser, and K. Kravarik for FISH advice, J. Wolfswinkel for FACS assistance. We thank X. Qiu and J. Chen for critically reading the manuscript. We thank all members of Jing lab for comments and discussion. We thank the staff in the core facility (Institute of Health Sciences) for technical assistance.?This work was supported in part by the National Key Research and Development Program of China (2017YFA0103700), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020903), and the National Natural Science Foundation of China (91739301, 91339205, and 31229002). ASCs, adult stem cells; dFISH, double fluorescent in situ hybridization; FACS, fluorescence-activated cell sorting; H3P, phosphorylated histone H3 at serine 10; RNAi, RNA interference; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; WISH, whole-mount in situ hybridization. Xiao-Shuai Han, Chen Wang, Fang-hao Guo, Shuang Huang, Yong-Wen Qin, Xian-Xian Zhao and Qing Jing declare that they have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/s13238-018-0512-0",
language = "English (US)",
volume = "10",
pages = "43--59",
journal = "Protein and Cell",
issn = "1674-800X",
publisher = "Springer-Verlag GmbH and Co. KG",
number = "1",
}