Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration

Xiao Shuai Han, Chen Wang, Fang hao Guo, Shuang Huang, Yong Wen Qin, Xian Xian Zhao, Qing Jing

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3′-5′ exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.

Original languageEnglish (US)
Pages (from-to)43-59
Number of pages17
JournalProtein and Cell
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Planarians
Zinc Fingers
Stem cells
Zinc
Regeneration
Cell proliferation
Proteins
RNA
Stem Cells
RNA Interference
Amputation
Animals
Genes
Tissue
Cell Proliferation
Defects
Adult Stem Cells
Wounds and Injuries
Phenotype

Keywords

  • Dis3l2
  • Schmidtea mediterranea
  • adult stem cells
  • local proliferation
  • planarians
  • wound recognition

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Drug Discovery
  • Cell Biology

Cite this

Han, X. S., Wang, C., Guo, F. H., Huang, S., Qin, Y. W., Zhao, X. X., & Jing, Q. (2019). Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration. Protein and Cell, 10(1), 43-59. https://doi.org/10.1007/s13238-018-0512-0

Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration. / Han, Xiao Shuai; Wang, Chen; Guo, Fang hao; Huang, Shuang; Qin, Yong Wen; Zhao, Xian Xian; Jing, Qing.

In: Protein and Cell, Vol. 10, No. 1, 01.01.2019, p. 43-59.

Research output: Contribution to journalArticle

Han, Xiao Shuai ; Wang, Chen ; Guo, Fang hao ; Huang, Shuang ; Qin, Yong Wen ; Zhao, Xian Xian ; Jing, Qing. / Neoblast-enriched zinc finger protein FIR1 triggers local proliferation during planarian regeneration. In: Protein and Cell. 2019 ; Vol. 10, No. 1. pp. 43-59.
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