Neogenin in amygdala for neuronal activity and information processing

Xiang Dong Sun, Wen Bing Chen, Dong Sun, Jie Huang, Yuan Quan Li, Jin Xiu Pan, Ya Nan Wang, Kai Zhao, Zhao Qi Dong, Hong Sheng Wang, Lei Xiong, Aiguo Xuan, Shen Ting Zhao, Anilkumar R Pillai, Wen Cheng Xiong, Lin Mei

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Fear learning and memory are vital for livings to survive, dysfunctions in which have been implicated in various neuropsychiatric disorders. Appropriate neuronal activation in amygdala is critical for fear memory. However, the underlying regulatory mechanisms are not well understood. Here we report that Neogenin, a DCC (deleted in colorectal cancer) family receptor, which plays important roles in axon navigation and adult neurogenesis, is enriched in excitatory neurons in BLA (Basolateral amygdala). Fear memory is impaired in male Neogenin mutant mice. The number of cFos+ neurons in response to tone-cued fear training was reduced in mutant mice, indicating aberrant neuronal activation in the absence of Neogenin. Electrophysiological studies show that Neogenin mutation reduced the cortical afferent input to BLA pyramidal neurons and compromised both induction and maintenance of Long-Term Potentiation evoked by stimulating cortical afferent, suggesting a role of Neogenin in synaptic plasticity. Concomitantly, there was a reduction in spine density and in frequency of miniature excitatory postsynaptic currents (mEPSCs), but not miniature inhibitory postsynaptic currents, suggesting a role of Neogenin in forming excitatory synapses. Finally, ablating Neogenin in the BLA in adult male mice impaired fear memory likely by reducing mEPSC frequency in BLA excitatory neurons. These results reveal an unrecognized function of Neogenin in amygdala for information processing by promoting and maintaining neurotransmission and synaptic plasticity and provide insight into molecular mechanisms of neuronal activation in amygdala.

Original languageEnglish (US)
Pages (from-to)9600-9613
Number of pages14
JournalJournal of Neuroscience
Volume38
Issue number44
DOIs
StatePublished - Oct 31 2018

Keywords

  • Amygdala
  • Fear memory
  • Neogenin
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Sun, X. D., Chen, W. B., Sun, D., Huang, J., Li, Y. Q., Pan, J. X., Wang, Y. N., Zhao, K., Dong, Z. Q., Wang, H. S., Xiong, L., Xuan, A., Zhao, S. T., Pillai, A. R., Xiong, W. C., & Mei, L. (2018). Neogenin in amygdala for neuronal activity and information processing. Journal of Neuroscience, 38(44), 9600-9613. https://doi.org/10.1523/JNEUROSCI.0433-18.2018