Abstract
Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1274-1280 |
| Number of pages | 7 |
| Journal | Diabetologia |
| Volume | 44 |
| Issue number | 10 |
| DOIs | |
| State | Published - Nov 9 2001 |
| Externally published | Yes |
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Keywords
- Beta cell
- Immunoglobulin superfamily
- Islets of Langerhans
- NPHS1 gene
- Nephrin
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
Cite this
Nephrin is expressed in the pancreatic beta cells. / Palmén, T.; Ahola, H.; Palgi, J.; Aaltonen, P.; Luimula, P.; Wang, Shixuan; Jaakkola, I.; Knip, M.; Otonkoski, T.; Holthöfer, H.
In: Diabetologia, Vol. 44, No. 10, 09.11.2001, p. 1274-1280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nephrin is expressed in the pancreatic beta cells
AU - Palmén, T.
AU - Ahola, H.
AU - Palgi, J.
AU - Aaltonen, P.
AU - Luimula, P.
AU - Wang, Shixuan
AU - Jaakkola, I.
AU - Knip, M.
AU - Otonkoski, T.
AU - Holthöfer, H.
PY - 2001/11/9
Y1 - 2001/11/9
N2 - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.
AB - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.
KW - Beta cell
KW - Immunoglobulin superfamily
KW - Islets of Langerhans
KW - NPHS1 gene
KW - Nephrin
UR - http://www.scopus.com/inward/record.url?scp=0034775038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034775038&partnerID=8YFLogxK
U2 - 10.1007/s001250100641
DO - 10.1007/s001250100641
M3 - Article
C2 - 11692176
AN - SCOPUS:0034775038
VL - 44
SP - 1274
EP - 1280
JO - Diabetologia
JF - Diabetologia
SN - 0012-186X
IS - 10
ER -