Nephrin is expressed in the pancreatic beta cells

T. Palmén, H. Ahola, J. Palgi, P. Aaltonen, P. Luimula, Shixuan Wang, I. Jaakkola, M. Knip, T. Otonkoski, H. Holthöfer

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

Original languageEnglish (US)
Pages (from-to)1274-1280
Number of pages7
JournalDiabetologia
Volume44
Issue number10
DOIs
StatePublished - Nov 9 2001
Externally publishedYes

Fingerprint

Insulin-Secreting Cells
Pancreas
Reverse Transcriptase Polymerase Chain Reaction
Fluorescent Antibody Technique
Glomerular Filtration Barrier
RNA
Keratin-19
Kidney
nephrin
Somatostatin
Glucagon
Islets of Langerhans
Gene Library
Proteinuria
Immunoblotting
Proteins
Complementary DNA
Messenger RNA
Antibodies

Keywords

  • Beta cell
  • Immunoglobulin superfamily
  • Islets of Langerhans
  • NPHS1 gene
  • Nephrin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Palmén, T., Ahola, H., Palgi, J., Aaltonen, P., Luimula, P., Wang, S., ... Holthöfer, H. (2001). Nephrin is expressed in the pancreatic beta cells. Diabetologia, 44(10), 1274-1280. https://doi.org/10.1007/s001250100641

Nephrin is expressed in the pancreatic beta cells. / Palmén, T.; Ahola, H.; Palgi, J.; Aaltonen, P.; Luimula, P.; Wang, Shixuan; Jaakkola, I.; Knip, M.; Otonkoski, T.; Holthöfer, H.

In: Diabetologia, Vol. 44, No. 10, 09.11.2001, p. 1274-1280.

Research output: Contribution to journalArticle

Palmén, T, Ahola, H, Palgi, J, Aaltonen, P, Luimula, P, Wang, S, Jaakkola, I, Knip, M, Otonkoski, T & Holthöfer, H 2001, 'Nephrin is expressed in the pancreatic beta cells', Diabetologia, vol. 44, no. 10, pp. 1274-1280. https://doi.org/10.1007/s001250100641
Palmén T, Ahola H, Palgi J, Aaltonen P, Luimula P, Wang S et al. Nephrin is expressed in the pancreatic beta cells. Diabetologia. 2001 Nov 9;44(10):1274-1280. https://doi.org/10.1007/s001250100641
Palmén, T. ; Ahola, H. ; Palgi, J. ; Aaltonen, P. ; Luimula, P. ; Wang, Shixuan ; Jaakkola, I. ; Knip, M. ; Otonkoski, T. ; Holthöfer, H. / Nephrin is expressed in the pancreatic beta cells. In: Diabetologia. 2001 ; Vol. 44, No. 10. pp. 1274-1280.
@article{0e9e90fcfb804ac0ba9b2347f4634448,
title = "Nephrin is expressed in the pancreatic beta cells",
abstract = "Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.",
keywords = "Beta cell, Immunoglobulin superfamily, Islets of Langerhans, NPHS1 gene, Nephrin",
author = "T. Palm{\'e}n and H. Ahola and J. Palgi and P. Aaltonen and P. Luimula and Shixuan Wang and I. Jaakkola and M. Knip and T. Otonkoski and H. Holth{\"o}fer",
year = "2001",
month = "11",
day = "9",
doi = "10.1007/s001250100641",
language = "English (US)",
volume = "44",
pages = "1274--1280",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Nephrin is expressed in the pancreatic beta cells

AU - Palmén, T.

AU - Ahola, H.

AU - Palgi, J.

AU - Aaltonen, P.

AU - Luimula, P.

AU - Wang, Shixuan

AU - Jaakkola, I.

AU - Knip, M.

AU - Otonkoski, T.

AU - Holthöfer, H.

PY - 2001/11/9

Y1 - 2001/11/9

N2 - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

AB - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

KW - Beta cell

KW - Immunoglobulin superfamily

KW - Islets of Langerhans

KW - NPHS1 gene

KW - Nephrin

UR - http://www.scopus.com/inward/record.url?scp=0034775038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034775038&partnerID=8YFLogxK

U2 - 10.1007/s001250100641

DO - 10.1007/s001250100641

M3 - Article

C2 - 11692176

AN - SCOPUS:0034775038

VL - 44

SP - 1274

EP - 1280

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 10

ER -