Nephrin is expressed in the pancreatic beta cells

T. Palmén; H. Ahola; J. Palgi; P. Aaltonen; P. Luimula; S. Wang; I. Jaakkola; M. Knip; T. Otonkoski; H. Holthöfer

doi:10.1007/s001250100641

Nephrin is expressed in the pancreatic beta cells

T. Palmén, H. Ahola, J. Palgi, P. Aaltonen, P. Luimula, S. Wang, I. Jaakkola, M. Knip, T. Otonkoski, H. Holthöfer

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 M_r protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

Original language	English (US)
Pages (from-to)	1274-1280
Number of pages	7
Journal	Diabetologia
Volume	44
Issue number	10
DOIs	https://doi.org/10.1007/s001250100641
State	Published - 2001
Externally published	Yes

Keywords

Beta cell
Immunoglobulin superfamily
Islets of Langerhans
NPHS1 gene
Nephrin

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism

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@article{0e9e90fcfb804ac0ba9b2347f4634448,

title = "Nephrin is expressed in the pancreatic beta cells",

abstract = "Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.",

keywords = "Beta cell, Immunoglobulin superfamily, Islets of Langerhans, NPHS1 gene, Nephrin",

author = "T. Palm{\'e}n and H. Ahola and J. Palgi and P. Aaltonen and P. Luimula and S. Wang and I. Jaakkola and M. Knip and T. Otonkoski and H. Holth{\"o}fer",

note = "Funding Information: Acknowledgements. This study was supported by the Helsinki University Hospital, the P{\"a}ivikki and Sakari Sohlberg Foundation, the Finnish Foundation of Diabetes Research and the Sigrid Juselius Foundation. T. Otonkoski is the recipient of a Juvenile Diabetes Foundation International Career Development Award. We are also indebted to L. Pirinen, E. H{\"a}yri and R. Majuri for their excellent practical assistance. In addition, we thank E. {\AA}str{\"o}m (M. Sci) and J. Ustinov (M. Sci) for their valuable help.",

year = "2001",

doi = "10.1007/s001250100641",

language = "English (US)",

volume = "44",

pages = "1274--1280",

journal = "Diabetologia",

issn = "0012-186X",

publisher = "Springer Verlag",

number = "10",

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TY - JOUR

T1 - Nephrin is expressed in the pancreatic beta cells

AU - Palmén, T.

AU - Ahola, H.

AU - Palgi, J.

AU - Aaltonen, P.

AU - Luimula, P.

AU - Wang, S.

AU - Jaakkola, I.

AU - Knip, M.

AU - Otonkoski, T.

AU - Holthöfer, H.

N1 - Funding Information: Acknowledgements. This study was supported by the Helsinki University Hospital, the Päivikki and Sakari Sohlberg Foundation, the Finnish Foundation of Diabetes Research and the Sigrid Juselius Foundation. T. Otonkoski is the recipient of a Juvenile Diabetes Foundation International Career Development Award. We are also indebted to L. Pirinen, E. Häyri and R. Majuri for their excellent practical assistance. In addition, we thank E. Åström (M. Sci) and J. Ustinov (M. Sci) for their valuable help.

PY - 2001

Y1 - 2001

N2 - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

AB - Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

KW - Beta cell

KW - Immunoglobulin superfamily

KW - Islets of Langerhans

KW - NPHS1 gene

KW - Nephrin

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JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 10

ER -

Nephrin is expressed in the pancreatic beta cells

Abstract

Keywords

ASJC Scopus subject areas

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