Nephrin is expressed in the pancreatic beta cells

T. Palmén, H. Ahola, J. Palgi, P. Aaltonen, P. Luimula, S. Wang, I. Jaakkola, M. Knip, T. Otonkoski, H. Holthöfer

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Aims/hypothesis. The NPHS1 gene product, nephrin, is a crucial component of the glomerular filtration barrier preventing proteinuria and previously assumed to be kidney-specific. The aim of this study was to describe the expression of nephrin mRNA and protein in human pancreas as well as identify the nephrin-expressing cell types. Methods. RNA dot blot, reverse transcriptase-polymerase chain reaction, sequencing, immunoblotting and dual immunofluorescence were used for the characterisation of nephrin in the pancreas. Results. Except for the kidney, the pancreas was found to be the only tissue expressing nephrin as screened with a human tissue RNA dot blot. The expression was verified with reverse transcriptase-polymerase chain reaction and by sequencing nephrin from a human pancreatic complementary DNA library. Nephrin antibody in immunoblot detected a 165000 Mr protein in the pancreas. Dual immunofluorescence showed that nephrin was specifically localised in the beta cells of the islets of Langerhans. There was no overlap with glucagon, somatostatin, or the ductal cell marker cytokeratin 19. Conclusion/interpretation. These data show that nephrin is a novel molecule of pancreatic beta cells.

Original languageEnglish (US)
Pages (from-to)1274-1280
Number of pages7
JournalDiabetologia
Volume44
Issue number10
DOIs
StatePublished - Nov 9 2001
Externally publishedYes

Keywords

  • Beta cell
  • Immunoglobulin superfamily
  • Islets of Langerhans
  • NPHS1 gene
  • Nephrin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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    Palmén, T., Ahola, H., Palgi, J., Aaltonen, P., Luimula, P., Wang, S., Jaakkola, I., Knip, M., Otonkoski, T., & Holthöfer, H. (2001). Nephrin is expressed in the pancreatic beta cells. Diabetologia, 44(10), 1274-1280. https://doi.org/10.1007/s001250100641